An integrated proteomic and physiological approach to understand the adhesion mechanism of the probiotic Lactobacillus reuteri Lb2 BM DSM 16143 (Articolo in rivista)

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Label
  • An integrated proteomic and physiological approach to understand the adhesion mechanism of the probiotic Lactobacillus reuteri Lb2 BM DSM 16143 (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.5584/jiomics.v2013i2013.143 (literal)
Alternative label
  • Erika Mangiapane1, Cristina Lamberti2, Alessandro Pessione1, Patrizia Ceruti3, Francesco Novelli3, Eugenio Galano4, Ritva Virkola5, Timo K. Korhonen5, Enrica Pessione1 (2013)
    An integrated proteomic and physiological approach to understand the adhesion mechanism of the probiotic Lactobacillus reuteri Lb2 BM DSM 16143
    in Journal of Integrated OMICS
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Erika Mangiapane1, Cristina Lamberti2, Alessandro Pessione1, Patrizia Ceruti3, Francesco Novelli3, Eugenio Galano4, Ritva Virkola5, Timo K. Korhonen5, Enrica Pessione1 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.jiomics.com/index.php/jio/article/view/143 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1.Department of Life Sciences and Systems Biology, Via Accademia Albertina 13, 10123, Torino University, Italy; 2.CNR ISPA, c/o Bioindustry Park S. Fumero, Via Ribes 5, 10010, Colleretto Giacosa, Italy; 3.Department of Medicine and Experimental Oncology, CeRMS, Via Cherasco 15, 10126, Torino University, Italy; 4.Department of Chemical Sciences, University of Naples \"Federico II\", Via Cinthia 4, 80126, Napoli, Italy; 5Division of General Microbiology Department of Biosciences, University of Helsinki, FIN-00014 Helsinki, Finland. (literal)
Titolo
  • An integrated proteomic and physiological approach to understand the adhesion mechanism of the probiotic Lactobacillus reuteri Lb2 BM DSM 16143 (literal)
Abstract
  • The adhesion ability of the probiotic Lactobacillus reuteri Lb2 BM DSM 16143 was tested to both enterocyte-like Caco-2 cells and to extracel- lular matrix proteins (laminin, fibronectin and collagen I and IV). The adhesiveness was lost after an alkaline treatment known to release moonlighting proteins from lactobacillar cell surface. To characterize the putative adhesive molecules, a 2-DE experiment in the pI range 4-7 was performed on the extracellular proteins. The expression of several moonlighting proteins involved in adhesion (i.e. GAPDH, EF-Tu, phosphoglycerate kinase) was demonstrated. Some of the identified adhesins were able to bind plasminogen (Plg), but did not convert it into plasmin (Plm), in absence of exogenous activators. This indicates that the moonlighting proteome of L. reuteri Lb2 BM DSM 16143 can con- tribute to adhesion processes. (literal)
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