Antigenic properties of HCMV peptides displayed by filamentous bacteriophages vs synthetic peptides (Articolo in rivista)

Type
Label
  • Antigenic properties of HCMV peptides displayed by filamentous bacteriophages vs synthetic peptides (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.imlet.2008.04.004 (literal)
Alternative label
  • Ulivieri C.; Citro A.; Ivaldi F.; Mascolo D.; Ghittoni R.; Fanigliulo D.; Manca F.; Baldari C.T.; Li Pira G. and Del Pozzo G. (2008)
    Antigenic properties of HCMV peptides displayed by filamentous bacteriophages vs synthetic peptides
    in Immunology letters
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Ulivieri C.; Citro A.; Ivaldi F.; Mascolo D.; Ghittoni R.; Fanigliulo D.; Manca F.; Baldari C.T.; Li Pira G. and Del Pozzo G. (literal)
Pagina inizio
  • 62 (literal)
Pagina fine
  • 70 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 119 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 1-2 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • CNR, Inst Genet & Biophys Adriano Buzzati Traverso, I-80131 Naples, Italy Univ Siena, Dept Evolutionary Biol, I-53100 Siena, Italy Adv Biotechnol Ctr, I-16132 Genoa, Italy Ist Giannina Gaslini, I-16148 Genoa, Italy Univ Siena, Dept Clin Med & Immunol Sci, I-53100 Siena, Italy (literal)
Titolo
  • Antigenic properties of HCMV peptides displayed by filamentous bacteriophages vs synthetic peptides (literal)
Abstract
  • Several efforts have been invested in the identification of CTL and Th epitopes, as well as in the characterization of their immunodominance and MHC restriction, for the generation of a peptide-based HCMV vaccine. Small synthetic peptides are, however, poor antigens and carrier proteins are important for improving the efficacy of synthetic peptide vaccines. Recombinant bacteriophages appear as promising tools in the design of subunit vaccines. To investigate the antigenicity of peptides carried by recombinant bacteriophages we displayed different HCMV MHCII restricted peptides on the capsid of filamentous bacteriophage (fd) and found that hybrid bacteriophages are processed by human APC and activate HCMV-specific CD4 T-cells. Furthermore we constructed a reporter T-cell hybridoma expressing a chimeric TCR comprising murine alpha beta constant regions and human variable regions specific for the HLA-A2 restricted immunodominant NLV peptide of HCMV. Using the filamentous bacteriophage as an epitope carrier, we detected a more robust and long lasting response of the reporter T-cell hybridoma compared to peptide stimulation. Our results show a general enhancement of T-cell responses when antigenic peptides are carried by phages. (literal)
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