http://www.cnr.it/ontology/cnr/individuo/prodotto/ID267530

Imaging of thyroid tumor angiogenesis with microbubbles targeted to vascular endothelial growth factor receptor type 2 in mice (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

Imaging of thyroid tumor angiogenesis with microbubbles targeted to vascular endothelial growth factor receptor type 2 in mice (Articolo in rivista) (literal)

Anno

2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1186/1471-2342-13-31 (literal)

Alternative label

Mancini M, Greco A, Salvatore G, Liuzzi R, and Di Maro, G. and Vergara, E. and Chiappetta, G. and Pasquinelli, R. and Brunetti, A. and Salvatore, M. (2013)
Imaging of thyroid tumor angiogenesis with microbubbles targeted to vascular endothelial growth factor receptor type 2 in mice
in BMC medical imaging (Online)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Mancini M, Greco A, Salvatore G, Liuzzi R, and Di Maro, G. and Vergara, E. and Chiappetta, G. and Pasquinelli, R. and Brunetti, A. and Salvatore, M. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

cited By (since 1996)0 (literal)

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http://www.scopus.com/inward/record.url?eid=2-s2.0-84883679160&partnerID=40&md5=341fcb1fbccdd22cc23cbe0c37dc65d7 (literal)

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13 (literal)

Rivista

BMC medical imaging (Rivista)

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31 (literal)

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1 (literal)

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Scopu (literal)

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a Institute of Biostructure and Bioimaging, Italian National Research Council (CNR), Naples, Italy b SDN Foundation IRCCS, Naples, Italy c Dipartimento di Scienze Biomediche Avanzate, Università degli Studi di Napoli Federico II, Naples, 80131, Italy (literal)

Titolo

Imaging of thyroid tumor angiogenesis with microbubbles targeted to vascular endothelial growth factor receptor type 2 in mice (literal)

Abstract

Background: To evaluate whether Contrast Enhanced Ultrasund (CEUS) with microbubbles (MBs) targeted to VEGFR-2 is able to characterize in vivo the VEGFR-2 expression in the tumor vasculature of a mouse model of thyroid cancer (Tg-TRK-T1). Methods: Animal protocol was approved by Institutional committee on Laboratory Animal Care. Contrast-enhanced ultrasound imaging with MBs targeted with an anti-VEGFR-2 monoclonal antibody (UCAVEGFR-2) and isotype control antibody (UCAIgG) was performed in 7 mice with thyroid carcinoma, 5 mice with hyperplasia or benign thyroid nodules and 4 mice with normal thyroid. After ultrasonography, the tumor samples were harvested for histological examination and VEGFR-2 expression was tested by immunohistochemistry. Data were reported as median and range. Paired non parametric Wilcoxon's test and ANOVA of Kruskal-Wallis were used. The correlation between the contrast signal and the VEGFR-2 expression was assessed by the Spearman coefficient. Results: The Video intensity difference (VID) caused by backscatter of the retained UCAVEGFR-2 was significantly higher in mice harboring thyroid tumors compared to mice with normal thyroids (P < 0.01) and to mice harboring benign nodules (P < 0.01). No statistically significant differences of VID were observed in the group of mice carrying benign nodules compared to mice with normal thyroids. Moreover in thyroid tumors VID of retained VEGFR-2-targeted UCA was significantly higher than that of control UCAIgG (P <0.05). Results of immunohistochemical analysis confirmed VEGFR-2 overexpression. The magnitude of the molecular ultrasonographic signal from a VEGFR-2-targeted UCA retained by tissue correlates with VEGFR-2 expression determined by immunohistochemistry (rho 0.793, P=0.0003). Conclusions: We demonstrated that CEUS with UCAVEGFR-2 might be used for in vivo non invasive detection and quantification of VEGFR-2 expression in thyroid cancer in mice, and to differentiate benign from malignant thyroid nodules (literal)

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