Adsorption and binding of AmpliTaq® DNA polymerase on the clay minerals, montmorillonite and kaolinite (Articolo in rivista)

Type
Label
  • Adsorption and binding of AmpliTaq® DNA polymerase on the clay minerals, montmorillonite and kaolinite (Articolo in rivista) (literal)
Anno
  • 1999-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/S0038-0717(98)00162-X (literal)
Alternative label
  • Vettori, C.a, Calamai, L.b, Yoder, M.c, Stotzky, G.d, Gallori, E.a (1999)
    Adsorption and binding of AmpliTaq® DNA polymerase on the clay minerals, montmorillonite and kaolinite
    in Soil biology & biochemistry; PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD (Regno Unito)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Vettori, C.a, Calamai, L.b, Yoder, M.c, Stotzky, G.d, Gallori, E.a (literal)
Pagina inizio
  • 587 (literal)
Pagina fine
  • 593 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 31 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
  • Scopu (literal)
  • Google Scholar (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • a Dept. of Animal Biology and Genetics, Univ. of Florence, Via Romana 17, 50125 Florence, Italy b Dept. of Soil Sci. and Plant Nutr., University of Florence, Florence, Italy c Electron Microscopy Facility, Dept. of Biol., New York University, New York, NY, United States (literal)
Titolo
  • Adsorption and binding of AmpliTaq® DNA polymerase on the clay minerals, montmorillonite and kaolinite (literal)
Abstract
  • Adsorption at equilibrium and binding of AmpliTaq(R) DNA polymerase (TDP) on clay minerals, two montmorillonites (W-M and Ap-M) and a kaolinite (K), were studied. Equilibrium adsorption isotherms were of the L type, and a plateau was reached with Ap-M and K, whereas no plateau was obtained with W-M. Adsorption of TDP was most rapid on W-M, maximal at pH 6 on all clays, and appeared to involve hydrophobic interactions between the clays and TDP. The amounts of TDP adsorbed and bound on the clays were higher on W-M than on Ap-M and K and occurred only on the external surfaces of the clays. Fourier-transform infrared spectra, SDS-PAGE and scanning electron microscopy of both pure TDP and the W-M-TDP complex showed the presence in the commercial TDP of microspheres (about 100 nm in diameter) of unidentified material, which could influence the adsorption-binding of TDP on clays. (literal)
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