Glucose-6-phosphate dehydrogenase plays a crucial role in protection from redox-stress-induced apoptosis (Articolo in rivista)

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  • Glucose-6-phosphate dehydrogenase plays a crucial role in protection from redox-stress-induced apoptosis (Articolo in rivista) (literal)
Anno
  • 2004-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1038/sj.cdd.4401420 (literal)
Alternative label
  • Fico A.; Paglialunga F.; Cigliano L.; Abrescia P.; Verde P.; Martini G.; Iaccarino I.; Filosa S. (2004)
    Glucose-6-phosphate dehydrogenase plays a crucial role in protection from redox-stress-induced apoptosis
    in Cell death and differentiation; Edward Arnold, Londra (Regno Unito)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Fico A.; Paglialunga F.; Cigliano L.; Abrescia P.; Verde P.; Martini G.; Iaccarino I.; Filosa S. (literal)
Pagina inizio
  • 823 (literal)
Pagina fine
  • 831 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 11 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 8 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • PUBBLICAZIONE (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • CNR, Inst Genet & Biophys Adriano Buzzati Traverso, I-80125 Naples, Italy Univ Naples Federico II, Dipartimento Fisiol Gen & Ambientale, I-80134 Naples, Italy (literal)
Titolo
  • Glucose-6-phosphate dehydrogenase plays a crucial role in protection from redox-stress-induced apoptosis (literal)
Abstract
  • Glucose-6-phosphate dehydrogenase-deleted embryonic stem (ES) cells (G6pdDelta) proliferate in vitro without special requirements, but when challenged with oxidants fail to sustain glutathione disulphide reconversion to reduced glutathione (GSH), entering a condition of oxidative stress. Here, we investigate the signalling events downstream of GSH oxidation in G6pdDelta and wild-type (wt) ES cells. We found that G6pdDelta ES cells are very sensitive to oxidants, activating an apoptotic pathway at oxidant concentrations otherwise sublethal for wt ES cells. We show that the apoptotic pathway activated by low oxidant concentrations is accompanied by mitochondria dysfunction, and it is therefore blocked by the overexpression of Bcl-X-L. Bcl-X-L does not inhibit the decrease in cellular GSH and reactive oxygen species formation following oxidant treatment. We also found that oxidant treatment in ES cells is followed by the activation of the MEK/ extracellular signal-regulated kinase (ERK) pathway. Interestingly, ERK activation has opposite outcomes in G6pdDelta ES cells compared to wt, which has a proapoptotic function in the first and a prosurvival function in the latter. We show that this phenomenon can be regulated by the cellular GSH level. (literal)
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