http://www.cnr.it/ontology/cnr/individuo/prodotto/ID26546
Biotechnological traps for the reduction of inflammation due to cardiopulmonary bypass operations. (Articolo in rivista)
- Type
- Label
- Biotechnological traps for the reduction of inflammation due to cardiopulmonary bypass operations. (Articolo in rivista) (literal)
- Anno
- 2006-01-01T00:00:00+01:00 (literal)
- Alternative label
Grano V, Salamino F, Melloni E, Minafra R, Regola E, Diano N, Nicolucci C, Attanasio A, Nappi G, Cotrufo M, Maresca L, De Santo NG, Mita DG (2006)
Biotechnological traps for the reduction of inflammation due to cardiopulmonary bypass operations.
in Biomaterials
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Grano V, Salamino F, Melloni E, Minafra R, Regola E, Diano N, Nicolucci C, Attanasio A, Nappi G, Cotrufo M, Maresca L, De Santo NG, Mita DG (literal)
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- aDepartment of Experimental Medicine, Biotechnology and Molecular Biology Section, Second University of Naples, Via S. M. di Costantinopoli 16, Naples 80138, Italy
bCNR A. Buzzati Traverso Institute of Genetics and Biophysics, Via P. Castellino 111, Naples 80131, Italy
cDepartment of Experimental Medicine, Biochemistry Section, University of Genoa, Viale Benedetto XV 1, Genoa 16132, Italy
dDepartment of Cardio-Thoracic and Respiratory Sciences, Second University of Naples, V. Monaldi Hospital, Naples 80100, Italy
eProfessor of Nephrology, Second University of Naples, Via Pansini, Naples 80100, Italy (literal)
- Titolo
- Biotechnological traps for the reduction of inflammation due to cardiopulmonary bypass operations. (literal)
- Abstract
- Cardiopulmonary bypass induces a systemic inflammatory response (SIR), characterized by the activation of cellular and humoral elements, with concomitant release of neutrophil elastase and matrix-metallo proteinases.
In the present study, the protease release during extracorporeal circulation in 28 patients undergoing cardiac surgical operations was monitored using casein zymography. A peak in protease activity was found in all patients at the end of cardiopulmonary bypass. Plasma samples of patients were allowed to interact with different traps obtained by immobilizing different protease inhibitors on specific carriers. ±1-Antitpypsin, Bovine Pancreatic Trypsin Inhibitor, Elastatinal or Leupeptin were used as inhibitors and were covalently immobilized by diazotization or by condensation. A reduction in the proteolytic activity of the plasma samples was observed after interaction with the different traps. The most efficient traps, i.e. the ones displaying greatest power to inhibit protease activity, were those obtained by immobilizing Bovine Pancreatic Trypsin Inhibitor and Leupeptin. The biocompatibility of traps was also tested.
Results show that protease activity in blood can be decreased by our protease traps. (literal)
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