Gene expression pathways induced by axotomy and decentralization of rat superior cervical ganglion neurons. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Gene expression pathways induced by axotomy and decentralization of rat superior cervical ganglion neurons. (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Alternative label

• Del Signore A, De Sanctis V, Di Mauro E, Negri R, Perrone-Capano C, Paggi P. (2006)
  Gene expression pathways induced by axotomy and decentralization of rat superior cervical ganglion neurons.
  in European journal of neuroscience (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Del Signore A, De Sanctis V, Di Mauro E, Negri R, Perrone-Capano C, Paggi P. (literal)

Pagina inizio

• 65 (literal)

Pagina fine

• 74 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 23 (literal)

Rivista

• European journal of neuroscience (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• * 1Dipartimento di Biologia Cellulare e dello Sviluppo, Università'La Sapienza', Piazzale A. Moro, 5, 00185 Roma, Italy; 2Fondazione 'Istituto Pasteur-Fondazione Cenci-Bolognetti' c/o Dipartimento di Genetica e Biologia Molecolare, Università'La Sapienza', Piazzale A. Moro, 5, 00185 Roma, ItalY; 3Istituto di Genetica e Biofisica 'A. Buzzati Traverso', CNR, Via Pietro Castellino 111, 80131 Napoli, Italy; 4Dipartimento di Farmacobiologia, Università degli Studi di Catanzaro 'Magna Graecia', Roccelletta di Borgia (CZ), Italy (literal)

Titolo

• Gene expression pathways induced by axotomy and decentralization of rat superior cervical ganglion neurons. (literal)

Abstract

• To identify genes potentially involved in remodelling synaptic connections, we induced the temporary detachment of pre- and post-synaptic elements by axotomy or denervation of rat superior cervical ganglion neurons. cDNA microarray analysis followed by stringent selection criteria allowed the identification of a panel of genes whose expression was modulated by axotomy at various time points after injury. Among these genes, 11 were validated by real-time reverse transcriptase-polymerase chain reaction on independently prepared samples after superior cervical ganglion neuron axotomy (1, 3 and 6 days) and compared with the effect of decentralization (8 h, 1 and 3 days). These genes code for extracellular matrix/space [apolipoprotein D (apoD), decorin, collagen alpha1 type I, collagen alpha1 type III] and intermediate filament (vimentin) proteins, for modulators of neurite outgrowth (thrombin receptor, plasminogen activator inhibitor-1, bone morphogenetic protein 4, annexin II and S-100-related protein, clone 42C) and for a nerve cell transcription factor (brain finger protein). Eight of these 11 genes showed significant and persistent modulations after both types of injury. Finally, protein levels of apoD were shown to increase in superior cervical ganglion after axotomy. Our results identify hitherto unrecorded genes responsive to axotomy and decentralization of superior cervical ganglion neurons, and probably involved in synapse formation, remodelling and elimination. (literal)

Prodotto di

• Meccanismi molecolari e cellulari della determinazione neurale, differenziamento neuronale e patologie del sistema nervoso (SV.P16.006.001) (Modulo)
• Institute of genetics and biophysics \"Adriano Buzzati Traverso\" (IGB) (Istituto)

Autore CNR

• CARLA PERRONE CAPANO (Unità di personale esterno)

Incoming links:

Prodotto

• Institute of genetics and biophysics \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Meccanismi molecolari e cellulari della determinazione neurale, differenziamento neuronale e patologie del sistema nervoso (SV.P16.006.001) (Modulo)

Autore CNR di

• CARLA PERRONE CAPANO (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• European journal of neuroscience (Rivista)