Phenotypes of naturally defective recb allele in Neisseria meningitidis clinical isolates (Articolo in rivista)

Type
Label
  • Phenotypes of naturally defective recb allele in Neisseria meningitidis clinical isolates (Articolo in rivista) (literal)
Anno
  • 2002-01-01T00:00:00+01:00 (literal)
Alternative label
  • Salvatore P., Bucci C., Pagliarulo C., Tredici M., Colicchio R., Cantalupo G., Bardaro M., Del Giudice L., Massardo D.R., Lavitola A., Bruni C.B., Alifano P (2002)
    Phenotypes of naturally defective recb allele in Neisseria meningitidis clinical isolates
    in Infection and immunity (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Salvatore P., Bucci C., Pagliarulo C., Tredici M., Colicchio R., Cantalupo G., Bardaro M., Del Giudice L., Massardo D.R., Lavitola A., Bruni C.B., Alifano P (literal)
Pagina inizio
  • 4185 (literal)
Pagina fine
  • 4195 (literal)
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  • 70 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Questo articolo mette in evidenza il fenotipo dell'allele recB difettivo in natura in isolati clinici di Neisseria meningitidis (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università di Napoli, Italy Centro di Endocrinologia ed Oncologia Sperimentale, CNR, Napoli, Italy Dipartimento di Scienze Ambientali, Seconda Università di Napoli, Caserta, Italy Dipartimento di Scienze e Tecnologie Biologiche e Ambientali, Università di Lecce, Italy Istituto di Genetica e Biofisica, CNR, Napoli, Italy (literal)
Titolo
  • Phenotypes of naturally defective recb allele in Neisseria meningitidis clinical isolates (literal)
Abstract
  • Neisseria meningitidis strains belonging to the hypervirulent lineage ET- 37 and several unrelated strains are extremely UV sensitive. The phenotype is consequent to the presence of a nonfunctional recBET-37 allele carrying multiple missense mutations. Phenotypic analysis has been performed with congenic menigococcal strains harboring either the wild-type recB allele or the recBET-37 allele. Congenic recBET-37 meningococci, in addition to being sensitive to UV, were defective both in repair of DNA lesions induced by UV treatment and partially, in recombination-mediated transformation. Consistently, the wild-type, but not the recBET-37, allele was able to complement the Escherichia coli recB21 mutation to UV resistance and proficiency in recombination. recBET-37 meningococci did not exhibit higher frequencies of spontaneous mutation to rifampin resistance than recB-proficient strains. However, mutation rates were enhanced following UV treatment, a phenomenon not observed in the recB- proficient counterpart. Interestingly, the results of PCR-based assays demonstrated that the presence of the recBET-37 allele considerably increased the frequency of recombination at the pilin loci. The main conclusion that can be drawn is that the presence of the defective recBET- 37 allele in N. meningitidis isolates causes an increase in genetic diversity, due to an ineffective RecBCD-dependent DNA repair and recombination pathway, and an increase in pilin antigenic variation. (literal)
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