Composition and Quantitation of Microalgal Lipids by ERETIC 1H NMR Method (Articolo in rivista)

Type
Label
  • Composition and Quantitation of Microalgal Lipids by ERETIC 1H NMR Method (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.3390/md11103742 (literal)
Alternative label
  • Genoveffa Nuzzo, Carmela Gallo, Giuliana d'Ippolito, Adele Cutignano, Angela Sardo and Angelo Fontana (2013)
    Composition and Quantitation of Microalgal Lipids by ERETIC 1H NMR Method
    in Marine drugs
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Genoveffa Nuzzo, Carmela Gallo, Giuliana d'Ippolito, Adele Cutignano, Angela Sardo and Angelo Fontana (literal)
Pagina inizio
  • 3742 (literal)
Pagina fine
  • 3753 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 11 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 12 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 10 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • CNR Istituto di Chimica Biomolecolare (literal)
Titolo
  • Composition and Quantitation of Microalgal Lipids by ERETIC 1H NMR Method (literal)
Abstract
  • Accurate characterization of biomass constituents is a crucial aspect of research in the biotechnological application of natural products. Here we report an efficient, fast and reproducible method for the identification and quantitation of fatty acids and complex lipids (triacylglycerols, glycolipids, phospholipids) in microalgae under investigation for the development of functional health products (probiotics, food ingredients, drugs, etc.) or third generation biofuels. The procedure consists of extraction of the biological matrix by modified Folch method and direct analysis of the resulting material by proton nuclear magnetic resonance (1H NMR). The protocol uses a reference electronic signal as external standard (ERETIC method) and allows assessment of total lipid content, saturation degree and class distribution in both high throughput screening of algal collection and metabolic analysis during genetic or culturing studies. As proof of concept, the methodology was applied to the analysis of three microalgal species (Thalassiosira weissflogii, Cyclotella cryptica and Nannochloropsis salina) which drastically differ for the qualitative and quantitative composition of their fatty acid-based lipids. (literal)
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