Positive-Negative Selection for homologous recombination in Arabidopsis. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Positive-Negative Selection for homologous recombination in Arabidopsis. (Articolo in rivista) (literal)

Anno

• 2001-01-01T00:00:00+01:00 (literal)

Alternative label

• Xiaohui Wang H, Viret JF, Eldridge A, Perera R, Signer ER, Chiurazzi M (2001)
  Positive-Negative Selection for homologous recombination in Arabidopsis.
  in Gene (Amst.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Xiaohui Wang H, Viret JF, Eldridge A, Perera R, Signer ER, Chiurazzi M (literal)

Pagina inizio

• 249 (literal)

Pagina fine

• 255 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 272 (literal)

Rivista

• Gene (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Genetica e Biofisica \"A. Buzzati Traberso Massachusetts Institute of Technology (literal)

Titolo

• Positive-Negative Selection for homologous recombination in Arabidopsis. (literal)

Abstract

• In plants gene knock-outs and targeted mutational analyses are hampered by the inefficiency of homologous recombination. We have developed a strategy to enrich for rare events of homologous recombination in Arabidopsis using combined positive and negative selection. The T-DNA targeting construct contained two flanking regions of the target alcohol dehydrogenase gene as homologous sequences, and neomycin phosphotransferase and cytosine deaminase as positive and negative markers, respectively. A root explant transformation procedure was used to obtain transgenic calli. Among 6250 transformants isolated by positive selection, 39 were found to be resistant to negative selection as well. Of these 39, at least one had undergone homologous recombination correlated with a unidirectional transfer of information. Although the ADH locus was not changed, our data demonstrate that a homologous recombination event can be selected by positive negative selection in plants. (literal)

Prodotto di

• Le interazioni benefiche fra organismi: dalla lotta biologica alla messa a punto di strumenti per un'agricoltura sostenibile. (AG.P01.005.001) (Modulo)
• Institute of genetics and biophysics \"Adriano Buzzati Traverso\" (IGB) (Istituto)

Autore CNR

• MAURIZIO CHIURAZZI (Unità di personale interno)

Incoming links:

Prodotto

• Institute of genetics and biophysics \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Le interazioni benefiche fra organismi: dalla lotta biologica alla messa a punto di strumenti per un'agricoltura sostenibile. (AG.P01.005.001) (Modulo)

Autore CNR di

• MAURIZIO CHIURAZZI (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Gene (Rivista)