Determination of the structure of seleno-methionine-labelled hydroxymethylbilane synthase in its active form by multi-wavelength anomalous dispersion (Articolo in rivista)

Type
Label
  • Determination of the structure of seleno-methionine-labelled hydroxymethylbilane synthase in its active form by multi-wavelength anomalous dispersion (Articolo in rivista) (literal)
Anno
  • 1999-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1107/S0907444998014711 (literal)
Alternative label
  • Hadener, A and Matzinger, PK and Battersby, AR and McSweeney, S and Thompson, AW and Hammersley, AP and Harrop, SJ and Cassetta, A and Deacon, A and Hunter, WN and Nieh, YP and Raftery, J and Hunter, N and Helliwell, JR (1999)
    Determination of the structure of seleno-methionine-labelled hydroxymethylbilane synthase in its active form by multi-wavelength anomalous dispersion
    in Acta crystallographica. Section D, Biological crystallography.; Blackwell, Oxford (Regno Unito)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Hadener, A and Matzinger, PK and Battersby, AR and McSweeney, S and Thompson, AW and Hammersley, AP and Harrop, SJ and Cassetta, A and Deacon, A and Hunter, WN and Nieh, YP and Raftery, J and Hunter, N and Helliwell, JR (literal)
Pagina inizio
  • 631 (literal)
Pagina fine
  • 643 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 55 (literal)
Rivista
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  • 3 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Univ Basel, Dept Chem, CH-4003 Basel, Switzerland; Hoffmann La Roche & Co Ltd, CH-4002 Basel, Switzerland; Univ Cambridge, Chem Lab, Cambridge CB2 1EW, England; SERC, Daresbury Lab, Warrington WA4 4AD, Cheshire, England; EMBL, ESRF, Grenoble, France; Univ Manchester, Dept Chem, Manchester M13 9PL, Lancs, England (literal)
Titolo
  • Determination of the structure of seleno-methionine-labelled hydroxymethylbilane synthase in its active form by multi-wavelength anomalous dispersion (literal)
Abstract
  • The enzyme hydroxymethylbilane synthase (HMBS, E.C. 4.3.1.8) catalyzes the conversion of porphobilinogen into hydroxymethylbilane, a key intermediate for the biosynthesis of heme, chlorophylls, vitamin Bit and related macrocycles. The enzyme is found in all organisms, except viruses. The crystal structure of the selenomethionine-labelled enzyme ([SeMet]HMBS) from Escherichia coli has been solved by the multi-wavelength anomalous dispersion (MAD) experimental method using the Daresbury SRS station 9.5. In addition, [SeMet]HMBS has been studied by MAD at the Grenoble ESRF MAD beamline BM14 (BL19) and this work is described especially with respect to the use of the ESRF CCD detector. The structure at ambient temperature has been refined, the R factor being 16.8% at 2.4 Angstrom resolution The dipyrromethane cofactor of the enzyme is preserved in its reduced form in the crystal and its geometrical shape is in full agreement with the crystal structures of authentic dipyrromethanes. Proximal to the reactive C atom of the reduced cofactor, spherical density is seen consistent with there being a water molecule ideally placed to take part in the final step of the enzyme reaction cycle. Intriguingly, the loop with residues 47-58 is not ordered in the structure of this form of the enzyme, which carries no substrate. Direct experimental study of the active enzyme is now feasible using time-resolved Laue diffraction and freeze-trapping, building on the structural work described here as the foundation. (literal)
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