http://www.cnr.it/ontology/cnr/individuo/prodotto/ID261776

Cobalt binding in the photosynthetic bacterium R. sphaeroides by X-ray absorption spectroscopy (Articolo in rivista)

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Label

Cobalt binding in the photosynthetic bacterium R. sphaeroides by X-ray absorption spectroscopy (Articolo in rivista) (literal)

Anno

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Alternative label

Belviso B.D., Italiano F., Caliandro R., Carrozzini B., Costanza, A. and Trotta M. (2013)
Cobalt binding in the photosynthetic bacterium R. sphaeroides by X-ray absorption spectroscopy
in BioMetals (Dordr., Online)
(literal)

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Belviso B.D., Italiano F., Caliandro R., Carrozzini B., Costanza, A. and Trotta M. (literal)

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Istituto di Cristallografia, Consiglio Nazionale delle Ricerche, Via Amendola 122/O, 70126 Bari, Italy Istituto per i Processi Chimico Fisici, Consiglio Nazionale delle Ricerche, Via Orabona 4, 70126 Bari, Italy Dipartimento di Biologia, Universita Aldo Moro di Bari, Via Orabona 4, 70126 Bari, Italy (literal)

Titolo

Cobalt binding in the photosynthetic bacterium R. sphaeroides by X-ray absorption spectroscopy (literal)

Abstract

Cobalt is an important oligoelement required for bacteria; if present in high concentration, exhibits toxic effects that, depending on the microor- ganism under investigation, may even result in growth inhibition. The photosynthetic bacterium Rhodobacter (R.) sphaeroides tolerates high cobalt concentration and bioaccumulates Co+2 ion, mostly on the cellular surface. Very little is known on the chemical fate of the bioaccumulated cobalt, thus an X-ray absorption spectroscopy investigation was conducted on R. sphaeroides cells to gain structural insights into the Co+2 binding to cellular components. X-ray absorption near-edge spectroscopy and extended X-ray absorption fine structure measurements were performed on R. sphaeroides samples containing whole cells and cell-free fractions obtained from cultures exposed to 5 mM Co+2. An octahedral coordination geometry was found for the cobalt ion, with six oxygen-ligand atoms in the first shell. In the soluble portion of the cell, cobalt was found bound to carboxylate groups, while a mixed pattern containing equivalent amount of two sulfur and two carbon atoms was found in the cell envelope fraction, suggesting the presence of carboxylate and sulfonate metal-binding functional groups, the latter arising from sulfolipids of the cell envelope. (literal)

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