AGRIS-FAO dbase: Partial characterization of a closterovirus associated with a chlorotic mottling of fig [Ficus carica L.; Apulia] (Banca dati)

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Label
  • AGRIS-FAO dbase: Partial characterization of a closterovirus associated with a chlorotic mottling of fig [Ficus carica L.; Apulia] (Banca dati) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Alternative label
  • Elbeaino; T.; Digiaro; M.; DE STRADIS A; Martelli; G.P. (2006)
    AGRIS-FAO dbase: Partial characterization of a closterovirus associated with a chlorotic mottling of fig [Ficus carica L.; Apulia]
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Elbeaino; T.; Digiaro; M.; DE STRADIS A; Martelli; G.P. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Source: Istituto di Servizi per il Mercato Agricolo Alimentare (Italy) ISMEA Via Cornelio Celso 6 00161 Rome Contact: Luca CALIGARA (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#titoloVolume
  • AGRIS 2013 - FAO of the United Nations (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#supporto
  • Altro (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • IVV - UOS BARI (literal)
Titolo
  • AGRIS-FAO dbase: Partial characterization of a closterovirus associated with a chlorotic mottling of fig [Ficus carica L.; Apulia] (literal)
Descrizione sintetica
  • AGRIS Categories Plant diseases; Plant genetics and breeding AGROVOC English terms Ficus carica; Closteroviruses; Leaves; Symptoms; Phloem; Rna; Dna; Pcr; Heat shock proteins; Genes; Molecular cloning; Nucleotide sequence; Amino acid sequences; Genetic distance; Disease surveys; Identification; Apulia (literal)
Abstract
  • A double-stranded RNA (dsRNA) ca. 19 Kbp in size was obtained from tissue extracts of a fig plant (Ficus carica) with chlorotic mottling and vein clearing of the leaves. Leaf dips showed the presence of filamentous closterovirus-like particles with distinct cross banding and a length of about 1,800 nm. No virus was recovered by mechanical inoculation to herbaceous hosts. However, virus aggregates were plentiful in thinsectioned companion cells and differentiating sieve tubes of symptomatic leaves. Using denatured dsRNA preparations as template, a 620 bp cDNA fragment was amplified by RT-PCR using degenerate primers designed on the phosphate motifs 1 and 2 of the heat shock-protein 70 homologue (HSP70h) of members of the family Closteroviridae. Computer-assisted analysis of the sequenced 620 bp fragment showed it to be part of a closteroviral HSP70h gene having identity at the amino acid level with the comparable gene of different closteroviral species ranging from 34 to 48. In a phylogenetic tree constructed with the amino acid sequences of part of the HSP70h of several members of the family Closteroviridae, the fig virus grouped with species of the genus Closterovirus. RT-PCR with specific primers designed on the HSP70h sequence detected the virus in 36 of 57 field-grown trees, most of which had mosaic symptoms. The present results show that fig hosts a putative closterovirus species for which the name of Fig leaf mottle-associated virus (FLMaV) is proposed (literal)
  • The AGRIS initiative was set up by the FAO in the 70s and created a worldwide cooperation for sharing access to agricultural science and technology information. Based on available technologies, AGRIS was initially collecting bibliographic references for a central database. However, since the advent of the Internet in the late 90s AGRIS has become the brand name for a network of centres, which are promoting the exchange of agricultural science and technology information through the use of common standards and methodologies. The AGRIS open archives and bibliographical databases cover the many aspects of agriculture, including forestry, animal husbandry, aquatic sciences and fisheries, and human nutrition, extension literature from over 100 participating countries. Material includes unique grey literature such as unpublished scientific and technical reports, theses, conference papers, government publications, and more. AGRIS today is part of the CIARD (Coherence in Information for Agricultural Research for Development) initiative, in which the CGIAR, GFAR and FAO collaborate to create a community for efficient knowledge sharing in agricultural research and development. We encourage all research centres or research information units all over the world to participate in this project. By contacting the AGRIS Secretariat all necessary information to participate will be provided. More information is available in the AGRIS wiki page. (literal)
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