The apolar distal histidine mutant (His69-->Val) of the homodimeric Scapharca hemoglobin is in an R-like conformation. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• The apolar distal histidine mutant (His69-->Val) of the homodimeric Scapharca hemoglobin is in an R-like conformation. (Articolo in rivista) (literal)

Anno

• 1998-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1021/bi972380f (literal)

Alternative label

• Guarrera L, Colotti G, Boffi A, Chiancone E, Das TK, Rousseau DL, Gibson QH. (1998)
  The apolar distal histidine mutant (His69-->Val) of the homodimeric Scapharca hemoglobin is in an R-like conformation.
  in Biochemistry (Easton); AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 (Stati Uniti d'America)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Guarrera L, Colotti G, Boffi A, Chiancone E, Das TK, Rousseau DL, Gibson QH. (literal)

Pagina inizio

• 5608 (literal)

Pagina fine

• 5615 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 37 (literal)

Rivista

• Biochemistry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 8 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 16 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Univ Roma La Sapienza, Dept Biochem Sci, CNR, Ctr Mol Biol, I-00185 Rome, Italy Albert Einstein Coll Med, Dept Physiol & Biophys, Bronx, NY 10461 USA Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77005 USA (literal)

Titolo

• The apolar distal histidine mutant (His69-->Val) of the homodimeric Scapharca hemoglobin is in an R-like conformation. (literal)

Abstract

• The effect of the apolar mutation of the distal histidine (His69-->Val) has been studied in the cooperative homodimeric hemoglobin from the mollusc Scapharca inaequivalvis, Absorption, circular dichroism, and resonance Raman spectroscopy point to a more symmetric heme structure of the deoxy derivative, which is indicative of an R-like conformation of the deoxy heme. Resonance Raman spectroscopy also brings out alterations in the geometry and interactions of the bound CO molecule. The iron-carbon stretching frequency is decreased by about 30 cm(-1) with respect to the native protein, while the diatomic ligand stretching frequency is increased by about the same degree, Consistent with the structural changes, the ligand binding properties are significantly altered, In the mutant the overall rate and the affinity for CO binding are increased about 100-fold with respect to the native protein, and cooperativity is abolished. Ln addition, the amplitude and the rate of the geminate rebinding process increase significantly. This finding may be correlated to the longer average residence time of the photolyzed CO molecule within the heme pocket of the H69V mutant, as indicated by molecular dynamics simulations. (literal)

Editore

• AMER CHEMICAL SOC (Editore)

Prodotto di

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Biologia strutturale e bioinformatica: struttura-funzione, dinamica e riconoscimento in proteine (SV.P14.008.001) (Modulo)

Autore CNR

• ALBERTO BOFFI (Unità di personale esterno)
• GIANNI COLOTTI (Unità di personale interno)
• EMILIA CHIANCONE (Unità di personale esterno)

Insieme di parole chiave

• Keywords of "The apolar distal histidine mutant (His69-->Val) of the homodimeric Scapharca hemoglobin is in an R-like conformation." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Biologia strutturale e bioinformatica: struttura-funzione, dinamica e riconoscimento in proteine (SV.P14.008.001) (Modulo)

Autore CNR di

• EMILIA CHIANCONE (Unità di personale esterno)
• GIANNI COLOTTI (Unità di personale interno)
• ALBERTO BOFFI (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biochemistry (Rivista)

Editore di

• AMER CHEMICAL SOC (Editore)

Insieme di parole chiave di

• Keywords of "The apolar distal histidine mutant (His69-->Val) of the homodimeric Scapharca hemoglobin is in an R-like conformation." (Insieme di parole chiave)