Beclomethasone dipropionate and formoterol reduce oxidative/nitrosative stress generated by cigarette smoke extracts and IL-17A in human bronchial epithelial cells. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Beclomethasone dipropionate and formoterol reduce oxidative/nitrosative stress generated by cigarette smoke extracts and IL-17A in human bronchial epithelial cells. (Articolo in rivista) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.ejphar.2013.08.001 (literal)

Alternative label

• Angela Marina Montalbano, Giulia Anzalone, Giusy Daniela Albano, Caterina Di Sano, Rosalia Gagliardo, Anna Bonanno, Loredana Riccobono, Gabriele Nicolini, Eleonora Ingrassia, Mark Gjomarkaj, Mirella Profita (2013)
  Beclomethasone dipropionate and formoterol reduce oxidative/nitrosative stress generated by cigarette smoke extracts and IL-17A in human bronchial epithelial cells.
  in European journal of pharmacology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Angela Marina Montalbano, Giulia Anzalone, Giusy Daniela Albano, Caterina Di Sano, Rosalia Gagliardo, Anna Bonanno, Loredana Riccobono, Gabriele Nicolini, Eleonora Ingrassia, Mark Gjomarkaj, Mirella Profita (literal)

Pagina inizio

• 418 (literal)

Pagina fine

• 427 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 718 (literal)

Rivista

• European journal of pharmacology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 10 (literal)

Note

• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• a Institute of Biomedicine and Molecular Immunology (IBIM), Italian National Research Council (CNR), Palermo, Italy b Dipartimento Biomedico di Medicina, Interna e Specialistica (Di.Bi.M.I.S.), Sezione di Pneumologia, University of Palermo, Palermo, Italy c Chiesi Farmaceutici SpA, Parma, Italy d Department of Experimental Biomedicine and Clinical Neuroscience, PhD Course in Experimental and Molecular Medicine, University of Palermo, Italy (literal)

Titolo

• Beclomethasone dipropionate and formoterol reduce oxidative/nitrosative stress generated by cigarette smoke extracts and IL-17A in human bronchial epithelial cells. (literal)

Abstract

• Interleukin-17A (IL-17A), cigarette smoke and oxidative/nitrosative stress are involved in inflammatory airway diseases, and the mechanisms behind these processes are still poorly understood. We investigated whether recombinant human IL-17A (rhIL-17A), in combination with cigarette smoke extracts (CSE), increases the levels of inducibile nitric oxide synthase (iNOS), reactive oxygen species, nitrotyrosine (NT) and the activation of signal transducer and activator of transcription 1 (STAT-1) in normal human bronchial epithelial cells (16HBE). The effect of beclomethasone dipropionate (BDP), formoterol and their combination was also evaluated. We demonstrated that rhIL-17A or CSE alone increases iNOS expression, reactive oxygen species and NT production and STAT-1 downstream signalling activation in terms of STAT-1ser727 and STAT-1tyr701 phosphorylation. The combination of both stimuli further increased iNOS, ROS, NT and STAT-1ser727 phosphorylation. The silencing of STAT-1 expression partially reduced the levels of iNOS, reactive oxygen species and NT generated by rhIL-17A and inhibited the effect of CSE alone in 16HBE cells. The treatment of the cells with the MEK1/2 inhibitor U0126 (1,4-diamino-2,3-dicyano-1,4-bis (o-aminophenylmercapto butadiene) abolished the expression of iNOS and STAT-1ser727 phosphorylation generated by rhIL-17A. 16HBE treated with BDP or formoterol alone partially suppressed the effect of IL-17A or CSE on ROS, NT, and STAT-1 activation. Furthermore the use of the drugs in combination showed an additive effect in 16HBE. Our findings demonstrate that IL-17A increases oxidative/nitrosative markers, likely via ERK1/2 downstream signalling and STAT-1 pathway activation in human bronchial epithelial cells. BDP and formoterol treatment reduces this effect showing an additive effect used in combination. (literal)

Prodotto di

• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)
• Modelli ex vivo/in vitro per lo studio dell'immunopatologia e della farmacologia nelle pneumopatie (ME.P01.014.007) (Modulo)

Autore CNR

• MIRELLA PROFITA (Unità di personale interno)
• ANNA BONANNO (Persona)
• giulia anzalone (Unità di personale esterno)
• ANGELA MARINA MONTALBANO (Persona)
• Giusy Daniela Albano (Unità di personale esterno)
• ROSALIA PAOLA GAGLIARDO (Unità di personale interno)
• CATERINA DI SANO (Unità di personale interno)
• LOREDANA RICCOBONO (Persona)
• MARK GJOMARKAJ (Persona)

Insieme di parole chiave

• Keywords of "Beclomethasone dipropionate and formoterol reduce oxidative/nitrosative stress generated by cigarette smoke extracts and IL-17A in human bronchial epithelial cells." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• MARK GJOMARKAJ (Persona)
• ANNA BONANNO (Persona)
• LOREDANA RICCOBONO (Persona)
• ROSALIA PAOLA GAGLIARDO (Unità di personale interno)
• CATERINA DI SANO (Unità di personale interno)
• MIRELLA PROFITA (Unità di personale interno)
• Giusy Daniela Albano (Unità di personale esterno)
• giulia anzalone (Unità di personale esterno)
• ANGELA MARINA MONTALBANO (Persona)

Prodotto

• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)
• Modelli ex vivo/in vitro per lo studio dell'immunopatologia e della farmacologia nelle pneumopatie (ME.P01.014.007) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• European journal of pharmacology (Rivista)

Insieme di parole chiave di

• Keywords of "Beclomethasone dipropionate and formoterol reduce oxidative/nitrosative stress generated by cigarette smoke extracts and IL-17A in human bronchial epithelial cells." (Insieme di parole chiave)