Amino acids in the capsid protein of TYLCV that are crucial for systemic infection, particle formation, and insect transmission (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Amino acids in the capsid protein of TYLCV that are crucial for systemic infection, particle formation, and insect transmission (Articolo in rivista) (literal)

Anno

• 1998-01-01T00:00:00+01:00 (literal)

Alternative label

• Noris E, Vaira AM, Caciagli P, Masenga V, Gronenborn B, Accotto GP. (1998)
  Amino acids in the capsid protein of TYLCV that are crucial for systemic infection, particle formation, and insect transmission
  in Journal of virology (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Noris E, Vaira AM, Caciagli P, Masenga V, Gronenborn B, Accotto GP. (literal)

Pagina inizio

• 10050 (literal)

Pagina fine

• 10057 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://jvi.asm.org/content/72/12/10050.full.pdf+html (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 72 (literal)

Rivista

• Journal of virology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 12 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• NE, VAM, CP, MV, AGP: IVV CNR, Str delle Cacce 73, 10135 Turin Italy; GB: ISV, CNRS, Avenue de la Terrasse, 11198 Gif sur Yvette, France (literal)

Titolo

• Amino acids in the capsid protein of TYLCV that are crucial for systemic infection, particle formation, and insect transmission (literal)

Abstract

• A functional capsid protein (CP) is essential for host plant infection and insect transmission in monopartite geminiviruses. We studied two defective genomic DNAs of tomato yellow leaf curl virus (TYLCV), Sic and SicRcv. Sie, cloned from a field-infected tomato, was not infectious, whereas SicRcv, which spontaneously originated from Sie, was infectious but not whitefly transmissible. A single amino acid change in the CP was found to be responsible for restoring infectivity. When the amino acid sequences of the CPs of Sic and SicRcv were compared with that of a closely related wild-type virus (TYLCV-Sar), differences were found in the following positions: 129 (P in Sic and SicRcv, Q in Sar), 134 (Q in Sic and Sar, H in SicRcv) and 152 (E in Sic and SicRcv, D in Sar). We constructed TYLCV-Sar variants containing the eight possible amino acid combinations in those three positions and tested them for infectivity and transmissibility. QQD, QQE, QHD, and QHE had a wild-type phenotype, whereas PHD and PHE were infectious but nontransmissible. PQD and PQE mutants were not infectious; however, they replicated and accumulated CP, but not virions, in Nicotiana benthamiana leaf discs. The Q129P replacement is a nonconservative change, which may drastically alter the secondary structure of the CP and affect its ability to form the capsid. The additional Q134H change, however, appeared to compensate for the structural modification. Sequence comparisons among whitefly-transmitted geminiviruses in terms of the CP region studied showed that combinations other than QQD are present in several cases, but never with a P129. (literal)

Autore CNR

• ANNA MARIA VAIRA (Unità di personale interno)
• GIAN PAOLO ACCOTTO (Unità di personale interno)
• VERA MASENGA (Persona)
• PIERO CACIAGLI (Unità di personale interno)
• EMANUELA NORIS (Unità di personale interno)

Incoming links:

Autore CNR di

• GIAN PAOLO ACCOTTO (Unità di personale interno)
• ANNA MARIA VAIRA (Unità di personale interno)
• PIERO CACIAGLI (Unità di personale interno)
• VERA MASENGA (Persona)
• EMANUELA NORIS (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of virology (Rivista)