A second rhodopsin-like protein in Cyanophora paradoxa: Gene sequence and protein expression in a cell-free system (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• A second rhodopsin-like protein in Cyanophora paradoxa: Gene sequence and protein expression in a cell-free system (Articolo in rivista) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Alternative label

• Frassanito AM, Barsanti L, Passarelli V, Evangelista V, Gualtieri P (2013)
  A second rhodopsin-like protein in Cyanophora paradoxa: Gene sequence and protein expression in a cell-free system
  in Journal of photochemistry and photobiology. B, Biology (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Frassanito AM, Barsanti L, Passarelli V, Evangelista V, Gualtieri P (literal)

Pagina inizio

• 188 (literal)

Pagina fine

• 193 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.sciencedirect.com/science/article/pii/S1011134413001310 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 125 (literal)

Rivista

• Journal of photochemistry and photobiology. B, Biology (Rivista)

Note

• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Biofisica, CNR, Via Moruzzi 1, 56124 Pisa, Italy (literal)

Titolo

• A second rhodopsin-like protein in Cyanophora paradoxa: Gene sequence and protein expression in a cell-free system (literal)

Abstract

• Here we report the identification and expression of a second rhodopsin-like protein in the alga Cyanophora paradoxa (Glaucophyta), named Cyanophopsin_2. This new protein was identified due to a serendipity event, since the RACE reaction performed to complete the sequence of Cyanophopsin_1, (the first rhodopsin-like protein of C. paradoxa identified in 2009 by our group), amplified a 619 bp sequence corresponding to a portion of a new gene of the same protein family. The full sequence consists of 1175 bp consisting of 849 bp coding DNA sequence and 4 introns of 326 bp. The protein is characterized by an N-terminal region of 47 amino acids, followed by a region with 7 ?-helices of 213 amino acids and a C-terminal region of 22 amino acids. This protein showed high identity with Cyanophopsin_1 and other rhodopsin-like proteins of Archea, Bacteria, Fungi and Algae. Cyanophosin_2 (CpR2) was expressed in a cell-free expression system, and characterized by means of absorption spectroscopy. (literal)

Prodotto di

• Istituto di biofisica (IBF) (Istituto)
• Biodispositivi e Biomolecole (MD.P01.020.001) (Modulo)

Autore CNR

• PAOLO GUALTIERI (Unità di personale interno)
• VALTERE EVANGELISTA (Unità di personale interno)
• LAURA BARSANTI (Persona)
• ANNA MARIA FRASSANITO (Unità di personale esterno)
• VINCENZO PASSARELLI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "A second rhodopsin-like protein in Cyanophora paradoxa: Gene sequence and protein expression in a cell-free system" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• VALTERE EVANGELISTA (Unità di personale interno)
• LAURA BARSANTI (Persona)
• VINCENZO PASSARELLI (Unità di personale interno)
• PAOLO GUALTIERI (Unità di personale interno)
• ANNA MARIA FRASSANITO (Unità di personale esterno)

Prodotto

• Istituto di biofisica (IBF) (Istituto)
• Biodispositivi e Biomolecole (MD.P01.020.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of photochemistry and photobiology. B, Biology (Rivista)

Insieme di parole chiave di

• Keywords of "A second rhodopsin-like protein in Cyanophora paradoxa: Gene sequence and protein expression in a cell-free system" (Insieme di parole chiave)