In planta production of a candidate vaccine against bovine papillomavirus type 1 (Articolo in rivista)

Type
Label
  • In planta production of a candidate vaccine against bovine papillomavirus type 1 (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1007/s00425-012-1692-0 (literal)
Alternative label
  • Love A.J., Chapman S.N., Matic S., Noris E., Lomonossoff G.P., Taliansky M. (2012)
    In planta production of a candidate vaccine against bovine papillomavirus type 1
    in Planta
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Love A.J., Chapman S.N., Matic S., Noris E., Lomonossoff G.P., Taliansky M. (literal)
Pagina inizio
  • 1305 (literal)
Pagina fine
  • 1313 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • First report of the production in plants of a candidate vaccine against Bovine papillomavirus 1, based on the expression of Virion like particles made of L1 protein. Funded by EU PLAPROVA KBBE-2008-227056 (literal)
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  • http://link.springer.com/content/pdf/10.1007%2Fs00425-012-1692-0.pdf (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 236 (literal)
Rivista
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  • 8 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 4 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • LAJ, CSN, TM James Hutton Inst Dundee, Dundee DD2 5DA, Scotland MS, NE CNR, Ist Virol Vegetale, I-10135 Turin, Italy LGP John Innes Ctr Plant Sci Res, Dept Biol Chem, Norwich NR4 7UH, Norfolk, England (literal)
Titolo
  • In planta production of a candidate vaccine against bovine papillomavirus type 1 (literal)
Abstract
  • Bovine papillomavirus type 1 (BPV-1) is an economically important virus that induces tumourigenic pathologies in horses and cows. Given that the BPV-1 L1 major coat protein can self-assemble into highly immunogenic higher-order structures, we transiently expressed it in Nicotiana benthamiana as a prelude to producing a candidate vaccine. It was found that plant codon optimization of L1 gave higher levels of expression than its non-optimized counterpart. Following protein extraction, we obtained high yields (183 mg/kg fresh weight leaf tissue) of relatively pure L1, which had self-assembled into virus-like particles (VLPs). We found that these VLPs elicited a highly specific and strong immune response, and therefore they may have utility as a potential vaccine. This is the first report demonstrating the viable production of a candidate BPV vaccine protein in plants. (literal)
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