http://www.cnr.it/ontology/cnr/individuo/prodotto/ID256700
Control of DNA Minor Groove Width and Fis Protein Binding by the Purine 2-amino Group (Articolo in rivista)
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- Control of DNA Minor Groove Width and Fis Protein Binding by the Purine 2-amino Group (Articolo in rivista) (literal)
- Anno
- 2013-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1093/nar/gkt357 (literal)
- Alternative label
Hancock S. P. [ 1 ] ; Ghane T. [ 2 ] ; Cascio D. [ 3 ] ; Rohs R. [ 4,5,6,7 ] ; Di Felice R. [ 2,4,6 ] ; Johnson R. C. [ 1,8 ] (2013)
Control of DNA Minor Groove Width and Fis Protein Binding by the Purine 2-amino Group
in Nucleic acids research; Oxford Abstracts Limited, OXFORD (Regno Unito)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Hancock S. P. [ 1 ] ; Ghane T. [ 2 ] ; Cascio D. [ 3 ] ; Rohs R. [ 4,5,6,7 ] ; Di Felice R. [ 2,4,6 ] ; Johnson R. C. [ 1,8 ] (literal)
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- [ 1 ] Univ Calif Los Angeles, David Geffen Sch Med, Dept Biol Chem, Los Angeles, CA 90095 USA
[ 2 ] CNR Inst Nanosci, Ctr S3, I-41125 Modena, Italy
[ 3 ] Univ Calif Los Angeles, Dept Energy, Inst Genom & Prote, Los Angeles, CA 90095 USA
[ 4 ] Univ So Calif, Mol & Computat Biol Program, Dept Biol Sci, Los Angeles, CA 90089 USA
[ 5 ] Univ So Calif, Dept Chem, Los Angeles, CA 90089 USA
[ 6 ] Univ So Calif, Dept Phys & Astron, Los Angeles, CA 90089 USA
[ 7 ] Univ So Calif, Dept Comp Sci, Los Angeles, CA 90089 USA
[ 8 ] Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90095 USA (literal)
- Titolo
- Control of DNA Minor Groove Width and Fis Protein Binding by the Purine 2-amino Group (literal)
- Abstract
- The width of the DNA minor groove varies with sequence and can be a major determinant of DNA shape recognition by proteins. For example, the minor groove within the center of the Fis-DNA complex narrows to about half the mean minor groove width of canonical B-form DNA to fit onto the protein surface. G/C base pairs within this segment, which is not contacted by the Fis protein, reduce binding affinities up to 2000-fold over A/T-rich sequences. We show here through multiple X-ray structures and binding properties of Fis-DNA complexes containing base analogs that the 2-amino group on guanine is the primary molecular determinant controlling minor groove widths. Molecular dynamics simulations of free-DNA targets with canonical and modified bases further demonstrate that sequence-dependent narrowing of minor groove widths is modulated almost entirely by the presence of purine 2-amino groups. We also provide evidence that protein-mediated phosphate neutralization facilitates minor groove compression and is particularly important for binding to non-optimally shaped DNA duplexes. (literal)
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