Glutamate neurotoxicity in rat cerebellar granule cells involves cytochrome c release from mitochondria and mitochondrial shuttle impairment. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Glutamate neurotoxicity in rat cerebellar granule cells involves cytochrome c release from mitochondria and mitochondrial shuttle impairment. (Articolo in rivista) (literal)

Anno

• 1999-01-01T00:00:00+01:00 (literal)

Alternative label

• ATLANTE A, GAGLIARDI S, MARRA E, CALISSANO P, PASSARELLA S (1999)
  Glutamate neurotoxicity in rat cerebellar granule cells involves cytochrome c release from mitochondria and mitochondrial shuttle impairment.
  in Journal of neurochemistry
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• ATLANTE A, GAGLIARDI S, MARRA E, CALISSANO P, PASSARELLA S (literal)

Pagina inizio

• 237 (literal)

Pagina fine

• 246 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 73 (literal)

Rivista

• Journal of neurochemistry (Rivista)

Note

• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Centro di Studio sui Mitocondri e Metabolismo Energetico, CNR - Bari; Dipartimento di Biochimica e Biologia Molecolare, Universita` di Bari, Bari; Istituto di Neurobiologia, CNR, Roma; Dipartimento di Scienze Animali, Vegetali e dell'Ambiente, Universita` del Molise, Campobasso, Italy (literal)

Titolo

• Glutamate neurotoxicity in rat cerebellar granule cells involves cytochrome c release from mitochondria and mitochondrial shuttle impairment. (literal)

Abstract

• To gain some insight into the mechanism by which glutamate neurotoxicity takes place in cerebellar granule cells, two steps of glucose oxidation were investigated: the electron flow via respiratory chain from certain substrates to oxygen and the transfer of extramitochondrial reducing equivalents via the mitochondrial shuttles. However, cytochrome c release from intact mitochondria was found to occur in glutamate-treated cells as detected photometrically in the supernatant of the cell homogenate suspension. As a result of cytochrome c release, an increase of the oxidation of externally added NADH was found, probably occurring via the NADH-b5 oxidoreductase of the outer mitochondrial membrane. When the two mitochondrial shuttles glycerol 3-phosphate/dihydroxyacetone phosphate and malate/oxaloacetate, devoted to oxidizing externally added NADH, were reconstructed, both were found to be impaired under glutamate neurotoxicity. Consistent early activation in two NADH oxidizing mechanisms, i.e., lactate production and plasma membrane NADH oxidoreductase activity, was found in glutamate-treated cells. In spite of this, the increase in the cell NADH fluorescence was found to be time-dependent, an index of the progressive damage of the cell. (literal)

Prodotto di

• Interrelazione nucleo/citoplasma/mitocondri nell'omeostasi cellulare. (SV.P15.003.001) (Modulo)
• Istituto di biomembrane e bioenergetica (IBBE) (Istituto)

Autore CNR

• ERSILIA MARRA (Unità di personale interno)
• ANNA ATLANTE (Unità di personale interno)
• PIETRO CALISSANO (Unità di personale esterno)

Incoming links:

Prodotto

• Istituto di biomembrane e bioenergetica (IBBE) (Istituto)
• Interrelazione nucleo/citoplasma/mitocondri nell'omeostasi cellulare. (SV.P15.003.001) (Modulo)

Autore CNR di

• ERSILIA MARRA (Unità di personale interno)
• ANNA ATLANTE (Unità di personale interno)
• PIETRO CALISSANO (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of neurochemistry (Rivista)