The ratio between the fast and slow forms of bovine cytochrome c oxidase is changed by cholate or nucleotides bound to the cholate-binding site close to the cytochrome a3/Cu(B) binuclear centre (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• The ratio between the fast and slow forms of bovine cytochrome c oxidase is changed by cholate or nucleotides bound to the cholate-binding site close to the cytochrome a3/Cu(B) binuclear centre (Articolo in rivista) (literal)

Anno

• 2000-01-01T00:00:00+01:00 (literal)

Alternative label

• Shoji K, Giuffrè A, D'Itri E, Hagiwara K, Yamanaka T, Brunori M, Sarti P (2000)
  The ratio between the fast and slow forms of bovine cytochrome c oxidase is changed by cholate or nucleotides bound to the cholate-binding site close to the cytochrome a3/Cu(B) binuclear centre
  in Cellular and molecular life sciences (Print. ed.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Shoji K, Giuffrè A, D'Itri E, Hagiwara K, Yamanaka T, Brunori M, Sarti P (literal)

Pagina inizio

• 1482 (literal)

Pagina fine

• 1487 (literal)

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• cited By (since 1996)2 (literal)

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• http://www.scopus.com/inward/record.url?eid=2-s2.0-0033774262&partnerID=40&md5=d14b74005cbe6868da5322c1a5e88370 (literal)

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• 57 (literal)

Rivista

• Cellular and molecular life sciences (Rivista)

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• 10 (literal)

Note

• Scopu (literal)

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• Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Kanda-Surugadai 1-5, Chiyoda-ku, Tokyo 101-8308, Japan; Department of Biochemical Sciences, CNR Center of Molecular Biology, University of Rome la Sapienza, Piazzale Aldo Moro 5, I-00185 Rome, Italy (literal)

Titolo

• The ratio between the fast and slow forms of bovine cytochrome c oxidase is changed by cholate or nucleotides bound to the cholate-binding site close to the cytochrome a3/Cu(B) binuclear centre (literal)

Abstract

• We determined the fraction of 'slow' and 'fast' conformations of bovine cytochrome c oxidase, following the kinetics of cyanide binding to the oxidized enzyme. We investigated whether treatment of heart mitochondrial particles with different commercially available types of cholate (standard and ultrapure) can affect the fraction of cytochrome c oxidase in the two states. Compared to standard cholate, the use of ultra-pure cholate for solubilization of heart mitochondrial particles significantly increased the fraction of the fast enzyme. Complete homogeneity (~ 100% fast) was observed when cytochrome c oxidase was solubilized with ultra-pure cholate from heart mitochondrial particles pre-equilibrated with AMP; equilibration with ADP yielded a much smaller fraction of fast enzyme (~ 35%). These observations are discussed on the basis of the structural relationships between the known cholate-binding site and the binuclear cytochrome a3-Cu(B) site: variation in the occupancy of this binding site with cholate or nucleotides may modify reactivity of the oxidized binuclear centre towards cyanide. (literal)

Prodotto di

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Biologia strutturale e bioinformatica: struttura-funzione, dinamica e riconoscimento in proteine (SV.P14.008.001) (Modulo)

Autore CNR

• MAURIZIO BRUNORI (Persona)
• ALESSANDRO GIUFFRE' (Unità di personale interno)

Insieme di parole chiave

• Keywords of "The ratio between the fast and slow forms of bovine cytochrome c oxidase is changed by cholate or nucleotides bound to the cholate-binding site close to the cytochrome a3/Cu(B) binuclear centre" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Biologia strutturale e bioinformatica: struttura-funzione, dinamica e riconoscimento in proteine (SV.P14.008.001) (Modulo)

Autore CNR di

• ALESSANDRO GIUFFRE' (Unità di personale interno)
• MAURIZIO BRUNORI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Cellular and molecular life sciences (Rivista)

Insieme di parole chiave di

• Keywords of "The ratio between the fast and slow forms of bovine cytochrome c oxidase is changed by cholate or nucleotides bound to the cholate-binding site close to the cytochrome a3/Cu(B) binuclear centre" (Insieme di parole chiave)