The heme-copper oxidases of Thermus thermophilus catalyze the reduction of nitric oxide: Evolutionary implications (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• The heme-copper oxidases of Thermus thermophilus catalyze the reduction of nitric oxide: Evolutionary implications (Articolo in rivista) (literal)

Anno

• 1999-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1073/pnas.96.26.14718 (literal)

Alternative label

• Giuffrè A, Stubauer G, Sarti P, Brunori M, Zumft WG, Buse G, Soulimane T (1999)
  The heme-copper oxidases of Thermus thermophilus catalyze the reduction of nitric oxide: Evolutionary implications
  in Proceedings of the National Academy of Sciences of the United States of America
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Giuffrè A, Stubauer G, Sarti P, Brunori M, Zumft WG, Buse G, Soulimane T (literal)

Pagina inizio

• 14718 (literal)

Pagina fine

• 14723 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• cited By (since 1996)96 (literal)

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• http://www.scopus.com/inward/record.url?eid=2-s2.0-0033593029&partnerID=40&md5=36480545c52758a8d40b0c053e553b48 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 96 (literal)

Rivista

• Proceedings of the National Academy of Sciences of the United States of America (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 26 (literal)

Note

• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Department of Biochemical Sciences, CNR Center of Molecular Biology, University of Rome La Sapienza, I-00185 Rome, Italy; Lehrstuhl für Mikrobiologie, Universität Fridericiana, D-76128 Karlsruhe, Germany; Institut für Biochemie, Rheinisch-Westfälische TH, D-52057 Aachen, Germany. (literal)

Titolo

• The heme-copper oxidases of Thermus thermophilus catalyze the reduction of nitric oxide: Evolutionary implications (literal)

Abstract

• We show that the heme-copper terminal oxidases of Thermus thermophilus (called ba3 and caa3) are able to catalyze the reduction of nitric oxide (NO) to nitrous oxide (N2O) under reducing anaerobic conditions. The rate of NO consumption and N2O production were found to be linearly dependent on enzyme concentration, and activity was abolished by enzyme denaturation. Thus, contrary to the eukaryotic enzyme, both T. thermophilus oxidases display a NO reductase activity (3.0 ± 0.7 mol NO/mol ba3 x min and 32 ± 8 mol NO/mol caa3 x min at [NO] ? 50 ?M and 20°C) that, though considerably lower than that of bona fide NO reductases (300-4,500 mol NO/mol enzyme x min), is definitely significant. We also show that for ba3 oxidase, NO reduction is associated to oxidation of cytochrome b at a rate compatible with turnover, suggesting a mechanism consistent with the stoichiometry of the overall reaction. We propose that the NO reductase activity of T. thermophilus oxidases may depend on a peculiar Cu(B)/+ coordination, which may be revealed by the forthcoming three-dimensional structure. These findings support the hypothesis of a common phylogeny of aerobic respiration and bacterial denitrification, which was proposed on the basis of structural similarities between the Pseudomonas stutzeri NO reductase and the cbb3 terminal oxidases. Our findings represent functional evidence in support of this hypothesis. (literal)

Prodotto di

• Biologia strutturale e bioinformatica: struttura-funzione, dinamica e riconoscimento in proteine (SV.P14.008.001) (Modulo)
• Institute of molecular biology and pathology (IBPM) (Istituto)

Autore CNR

• MAURIZIO BRUNORI (Persona)
• ALESSANDRO GIUFFRE' (Unità di personale interno)

Insieme di parole chiave

• Keywords of "The heme-copper oxidases of Thermus thermophilus catalyze the reduction of nitric oxide: Evolutionary implications" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Biologia strutturale e bioinformatica: struttura-funzione, dinamica e riconoscimento in proteine (SV.P14.008.001) (Modulo)

Autore CNR di

• ALESSANDRO GIUFFRE' (Unità di personale interno)
• MAURIZIO BRUNORI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Proceedings of the National Academy of Sciences of the United States of America (Rivista)

Insieme di parole chiave di

• Keywords of "The heme-copper oxidases of Thermus thermophilus catalyze the reduction of nitric oxide: Evolutionary implications" (Insieme di parole chiave)