Purification and characterization of recombinant human 5'-methylthioadenosine phosphorylase: Definite identification of coding cDNA (Articolo in rivista)

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  • Purification and characterization of recombinant human 5'-methylthioadenosine phosphorylase: Definite identification of coding cDNA (Articolo in rivista) (literal)
Anno
  • 1996-01-01T00:00:00+01:00 (literal)
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  • 10.1006/bbrc.1996.0926 (literal)
Alternative label
  • Della Ragione F., Takabayashi K., Mastropietro S., Mercurio C., Oliva A., Russo G.L., Della Pietra V., Borriello A., Nobori T., Carson D.A., Zappia V. (1996)
    Purification and characterization of recombinant human 5'-methylthioadenosine phosphorylase: Definite identification of coding cDNA
    in Biochemical and biophysical research communications (Print); Elsevier Inc., San Diego (Stati Uniti d'America)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Della Ragione F., Takabayashi K., Mastropietro S., Mercurio C., Oliva A., Russo G.L., Della Pietra V., Borriello A., Nobori T., Carson D.A., Zappia V. (literal)
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  • 514 (literal)
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  • 519 (literal)
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  • cited By (since 1996)6 (literal)
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  • 223 (literal)
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  • 6 (literal)
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  • 3 (literal)
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  • Inst. of Biochem. of Macromolecules, Medical School, Second University of Naples, Naples, Italy; C.N.R., Inst. of Food Sci. and Technology, Avellino, Italy; CIBA-GEIGY Pharmaceutical Division, CH-4002 Basel, Switzerland; Department of Medicine, School of Medicine, University of California, San Diego, CA, United States; Stazione Zoologica Anton Dohrn, Villa Comunale 1, 80121 Naples, Italy (literal)
Titolo
  • Purification and characterization of recombinant human 5'-methylthioadenosine phosphorylase: Definite identification of coding cDNA (literal)
Abstract
  • 5'-Methylthioadenosine phosphorylase gene maps on the 9p21 chromosome, strictly linked to the important tumor suppressor gene p16(INK4A). Chromosomal deletions encompassing both the phosphorylase and p16(INK4A) genes cause the complete absence of the enzymatic activity in a large number of tumors, thus resulting in well-defined metabolic differences between malignant and normal cells. Recently, the cloning of the phosphorylase gene has been reported on the basis of indirect evidence. In order to demonstrate definitely the identification of 5'-methylthioadenosine phosphorylase gene, we have cloned the putative enzyme coding sequence in a prokaryotic expression vector and expressed the protein in bacteria. The recombinant phosphorylase has been purified to homogeneity and its physicochemical, immunological and kinetic features have been characterized. The results obtained allowed the conclusive demonstration of 5'-methylthioadenosine phosphorylase gene cloning and the use of recombinant protein for further characterization. (literal)
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