AFM structural study of the molecular chaperone GroEL and its two-dimensional crystals: an ideal \"living\" calibration sample (Articolo in rivista)

Type
Label
  • AFM structural study of the molecular chaperone GroEL and its two-dimensional crystals: an ideal \"living\" calibration sample (Articolo in rivista) (literal)
Anno
  • 2002-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/S0304-3991(02)00149-3 (literal)
Alternative label
  • Valle, F; DeRose, JA; Dietler, G; Kawe, M; Pluckthun, A; Semenza, G (2002)
    AFM structural study of the molecular chaperone GroEL and its two-dimensional crystals: an ideal "living" calibration sample
    in Ultramicroscopy (Amst.); North Holland Pub. Co., Amsterdam (Paesi Bassi)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Valle, F; DeRose, JA; Dietler, G; Kawe, M; Pluckthun, A; Semenza, G (literal)
Pagina inizio
  • 83 (literal)
Pagina fine
  • 89 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 93 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 7 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 1 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • [ 1 ] Univ Lausanne, BSP, Inst Phys Matiere Condensee, CH-1015 Lausanne, Switzerland [ 2 ] Univ Zurich, Inst Biochem, CH-8057 Zurich, Switzerland [ 3 ] ETH Zentrum, Swiss Fed Inst Technol, Inst Biochem, CH-8092 Zurich, Switzerland [ 4 ] Univ Milan, Inst Biochim, I-20133 Milan, Italy (literal)
Titolo
  • AFM structural study of the molecular chaperone GroEL and its two-dimensional crystals: an ideal \"living\" calibration sample (literal)
Abstract
  • Supramolecular complexes, such as chaperonins, are suitable samples for atomic force microscope structural studies because they have a very well defined shape. High-resolution images can be made using tapping mode in liquid under native conditions. Details about the two-dimensional structures formed onto the surface upon adsorption and of the single protein can be observed. Dissection of the upper ring of the supramolecular complex as a result of the applied lateral force through scanning tip is observed. Finally, the combination of lateral convolution and tip penetration into the cavity of chaperonins offers a direct evaluation of the tip convolution effect on images of macromolecular samples. (literal)
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