http://www.cnr.it/ontology/cnr/individuo/prodotto/ID251637
AFM structural study of the molecular chaperone GroEL and its two-dimensional crystals: an ideal \"living\" calibration sample (Articolo in rivista)
- Type
- Label
- AFM structural study of the molecular chaperone GroEL and its two-dimensional crystals: an ideal \"living\" calibration sample (Articolo in rivista) (literal)
- Anno
- 2002-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/S0304-3991(02)00149-3 (literal)
- Alternative label
Valle, F; DeRose, JA; Dietler, G; Kawe, M; Pluckthun, A; Semenza, G (2002)
AFM structural study of the molecular chaperone GroEL and its two-dimensional crystals: an ideal "living" calibration sample
in Ultramicroscopy (Amst.); North Holland Pub. Co., Amsterdam (Paesi Bassi)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Valle, F; DeRose, JA; Dietler, G; Kawe, M; Pluckthun, A; Semenza, G (literal)
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- [ 1 ] Univ Lausanne, BSP, Inst Phys Matiere Condensee, CH-1015 Lausanne, Switzerland
[ 2 ] Univ Zurich, Inst Biochem, CH-8057 Zurich, Switzerland
[ 3 ] ETH Zentrum, Swiss Fed Inst Technol, Inst Biochem, CH-8092 Zurich, Switzerland
[ 4 ] Univ Milan, Inst Biochim, I-20133 Milan, Italy (literal)
- Titolo
- AFM structural study of the molecular chaperone GroEL and its two-dimensional crystals: an ideal \"living\" calibration sample (literal)
- Abstract
- Supramolecular complexes, such as chaperonins, are suitable samples for atomic force microscope structural studies because they have a very well defined shape. High-resolution images can be made using tapping mode in liquid under native conditions. Details about the two-dimensional structures formed onto the surface upon adsorption and of the single protein can be observed. Dissection of the upper ring of the supramolecular complex as a result of the applied lateral force through scanning tip is observed. Finally, the combination of lateral convolution and tip penetration into the cavity of chaperonins offers a direct evaluation of the tip convolution effect on images of macromolecular samples. (literal)
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