Evolution of a Lolium rigidum population resistant to ACCase and ALS inhibitors in wheat: biochemical characterisation of resistance mechanisms (Abstract/Comunicazione in atti di convegno)

Type
Label
  • Evolution of a Lolium rigidum population resistant to ACCase and ALS inhibitors in wheat: biochemical characterisation of resistance mechanisms (Abstract/Comunicazione in atti di convegno) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Alternative label
  • Collavo A., Beffa R., Sattin M. (2013)
    Evolution of a Lolium rigidum population resistant to ACCase and ALS inhibitors in wheat: biochemical characterisation of resistance mechanisms
    in Conference "Global herbicide resistance challange", Perth (Australia), 18-22 febbraio 2013
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Collavo A., Beffa R., Sattin M. (literal)
Note
  • Comunicazione (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • IBAF-CNR Bayer CropScience (literal)
Titolo
  • Evolution of a Lolium rigidum population resistant to ACCase and ALS inhibitors in wheat: biochemical characterisation of resistance mechanisms (literal)
Abstract
  • ACCase-resistant Lolium spp. is spread worldwide in many crops, leading growers to often adopt ALS inhibitors to control the resistant populations. Consequently, intensive use of ALS inhibitors can generate resistance to both ACCase and ALS inhibitors which will present a serious challenge to wheat growers. To evaluate the long-term efects of ACCase-resistant L. rigidum management strategies based on the use of graminicidal sulfonylureas (SUs), a seven-year experiment was set up. The strategies included the continued use of ALS inhibitors, the continued application of ACCase inhibitors, and a resistance management strategy based on a biennial mode of action rotation between ALS inhibitors and herbicides with other modes of action diferent from ACCase. Lolium rigidum seed samples were collected each year from the survivors in each experimental plot and analysed using bioassays. Then target site resistance and enhanced metabolic resistance were assessed using, respectively, pyrosequencing and HPLC analyses. ALS inhibitors controlled the resistant L. rigidum until the third application. Then the efcacy started to decline at diferent levels depending on the treatment program used, and multi-resistant plants were observed at diferent rates but in all the tested strategies. Pyrosequencing analysis revealed fve diferent ACCase mutations, while none of the tested plants displayed amino acid substitutions at positions 197 or 574 of the ALS gene. Analyses of mesosulfuron metabolism showed a signifcantly higher level of metabolites in plants that originated from seeds collected at the end of the experiment when ALS inhibitors were used. The metabolism of ACCase inhibitors was found to be rather low. The change from continuous use of ACCase inhibitors to continuous use of ALS- inhibiting herbicides appears a short-term tactic, whereas a simple resistance management strategy based on a biennial rotation between SUs and other modes of action delays the selection of multiple resistance, but is not sustainable in the medium- to long-term without the additional use of other integrated weed management tools. (literal)
Editore
Prodotto di
Autore CNR

Incoming links:


Prodotto
Autore CNR di
Editore di
data.CNR.it