The tbf-1 gene from the white truffle Tuber borchii codes for a structural cell wall protein specifically expressed in fruitbody. (Articolo in rivista)

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  • The tbf-1 gene from the white truffle Tuber borchii codes for a structural cell wall protein specifically expressed in fruitbody. (Articolo in rivista) (literal)
Anno
  • 1998-01-01T00:00:00+01:00 (literal)
Alternative label
  • DE BELLIS R, AGOSTINI D, PICCOLI G, VALLORANI L, POTENZA L, POLIDORI E, SISTI D, AMORESANO A, PUCCI P, ARPAIA G, MACINO G, BALESTRINI R., BONFANTE P, STOCCHI V (1998)
    The tbf-1 gene from the white truffle Tuber borchii codes for a structural cell wall protein specifically expressed in fruitbody.
    in Fungal genetics and biology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • DE BELLIS R, AGOSTINI D, PICCOLI G, VALLORANI L, POTENZA L, POLIDORI E, SISTI D, AMORESANO A, PUCCI P, ARPAIA G, MACINO G, BALESTRINI R., BONFANTE P, STOCCHI V (literal)
Pagina inizio
  • 87 (literal)
Pagina fine
  • 99 (literal)
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  • 25 (literal)
Rivista
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  • Istituto di Chimica Biologica ''Giorgio Fornaini,'' Via Saffi 2, 61029 Urbino (PS), Italy; Istituto Orto Botanico, Via Bramante 28, 61029 Urbino (PS), Italy; Servizio di Spettrometria di Massa, Via Pansini 5, 80131 Naples, Italy; Dip. di Biopatologia Umana, Policlinico Umberto I, Viale Regina Elena 324, 00161 Rome, Italy; and Centro di Micologia del Terreno, Via Mattioli 25, 10125 Turin, Italy (literal)
Titolo
  • The tbf-1 gene from the white truffle Tuber borchii codes for a structural cell wall protein specifically expressed in fruitbody. (literal)
Abstract
  • This paper reports the purification and localization of a Tuber borchii Vittad. fruitbody protein (TBF-1) and the cloning of the encoding gene. TBF-1 is detectable by SDS-PAGE analyses only in this white truffle species and presents a molecular mass of 11,994 Da. TBF-1 was purified by one-step Reversed-Phase HPLC and its complete amino acid sequence was determined after digestion with trypsin and N-Asp endoproteinase. Polyclonal antibodies were produced and tested in immunofluorescence and immunogold experiments, providing information about the protein localization. It was detected mostly on the hyphal walls, where it was colocalized with b-1,3-glucans and chitin. The sporal wall was not labeled. The encoding gene (tbf-1) was cloned using several techniques involving PCR. The coding region consists of a 360-bp open reading frame interrupted by an intron, with another intron following the stop codon. A putative signal peptide of 12 amino acids was found at the N-terminal. Northern blot analysis revealed that tbf-1 is highly expressed in unripe and ripe fruitbodies and was not detectable in culture mycelium or ectomycorrhizal roots. (literal)
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