Eucalypt NADP-dependent isocitrate dehydrogenase: cDNA cloning and expression in ectomycorrhizae. (Articolo in rivista)

Type
Label
  • Eucalypt NADP-dependent isocitrate dehydrogenase: cDNA cloning and expression in ectomycorrhizae. (Articolo in rivista) (literal)
Anno
  • 1998-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1104/pp.117.3.939 (literal)
Alternative label
  • BOIFFIN V, HODGES H, GALVEZ S, BALESTRINI R., BONFANTE P, GADAL P, MARTIN F (1998)
    Eucalypt NADP-dependent isocitrate dehydrogenase: cDNA cloning and expression in ectomycorrhizae.
    in Plant physiology (Bethesda)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • BOIFFIN V, HODGES H, GALVEZ S, BALESTRINI R., BONFANTE P, GADAL P, MARTIN F (literal)
Pagina inizio
  • 939 (literal)
Pagina fine
  • 948 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 117 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Equipe de Microbiologie Forestiere, Institut National de la Recherche Agronomique, Centre de Nancy, F-54280 Champenoux, France (V.B., F.M.); Institut de Biotechnologie des Plantes, Centre National de la Recherche Scientifique ERS 569, Ba^timent 630, Universite´ Paris-Sud, F-91405 Orsay ce´dex, France (V.B., M.H., S.G., P.G.); and Dipartimento di Biologia Vegetale dell' Universita', Centro di Studio sulla Micologia del Terreno, del Centro Nazionale de Ricerca, Viale Mattioli 25, I-10125 Torino, Italy (R.B., P.B.) (literal)
Titolo
  • Eucalypt NADP-dependent isocitrate dehydrogenase: cDNA cloning and expression in ectomycorrhizae. (literal)
Abstract
  • NADP-dependent isocitrate dehydrogenase (NADP-ICDH) activity is increased in roots of Eucalyptus globulus subsp. bicostata ex Maiden Kirkp. during colonization by the ectomycorrhizal fungus Pisolithus tinctorius Coker and Couch. To investigate the regulation of the enzyme expression, a cDNA (EgIcdh) encoding the NADPICDH was isolated from a cDNA library of E. globulus-P. tinctorius ectomycorrhizae. The putative polypeptide sequence of EgIcdh showed a high amino acid similarity with plant NADP-ICDHs. Because the deduced EgICDH protein lacks an amino-terminal targeting sequence and shows highest similarity to plant cytosolic ICDHs, it probably represents a cytoplasmic isoform. RNA analysis showed that the steady-state level of EgIcdh transcripts was enhanced nearly 2-fold in ectomycorrhizal roots compared with nonmycorrhizal roots. Increased accumulation of NADP-ICDH transcripts occurred as early as 2 d after contact and likely led to the observed increased enzyme activity. Indirect immunofluorescence microscopy indicated that NADP-ICDH was preferentially accumulated in the epidermis and stele parenchyma of nonmycorrhizal and ectomycorrhizal lateral roots. The putative role of cytosolic NADP-ICDH in ectomycorrhizae is discussed. (literal)
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