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Genetic variability among flavescence doree phytoplasmas from different origins in Italy and France (Articolo in rivista)

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Genetic variability among flavescence doree phytoplasmas from different origins in Italy and France (Articolo in rivista) (literal)

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Alternative label

Martini, M., Botti, S., Marcone, C., Marzachi, C., Casati, P., Bianco, P.A., Benedetti, R., Bertaccini, A (2002)
Genetic variability among flavescence doree phytoplasmas from different origins in Italy and France
in Molecular and cellular probes (Print); ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, LONDON (Regno Unito)
(literal)

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Martini, M., Botti, S., Marcone, C., Marzachi, C., Casati, P., Bianco, P.A., Benedetti, R., Bertaccini, A (literal)

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Note

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MM, BS, BA: Università di Bologna MC: Università della Basilicata MC: Istituto di Virologia Vegetale, CNR, Torino, Italia CP, BPA: Università di Milano BR: Università di Udine (literal)

Titolo

Genetic variability among flavescence doree phytoplasmas from different origins in Italy and France (literal)

Abstract

Flavescence doree is a devastating disease of grapevine widespread in several countries in EU such as France, Italy and Spain. Genetic variability among 17 Italian and 3 French FD strains was investigated by RFLP analyses based on a fragment of the ribosomal protein operon and on the non-ribosomal DNA fragment FD9. RFLP analysis of the PCR amplified ribosomal protein fragment, coding for the 3' end of rpl22 and the entire rps3 genes, differentiated 4 rp-subgroups among the FD strains and 4 subgroups among the reference strains belonging to elm yellows group (16SrV). Sequencing and phylogenetic analysis of the same ribosomal protein DNA fragment validated the delineation of 4 distinct FD strain types derived by RFLP analyses. The results supported the differentiation based on analysis of the non-ribosomal DNA fragment FD9. The phylogenetic analysis further revealed relationships and a probable evolutionary trend among the FD strains and the other representatives of elm yellows group. All the FD strains together with the reference strains ALY, RuS and JWB formed a cluster very well distinct from the EY/ULW cluster. Moreover, ALY was shown to be more closely related to three FD strain types: the Lombardia/Piemonte, the French FD70, and the French FD88/ltalian FD-D strain clusters. (C) 2002 Elsevier Science Ltd. All rights reserved. (literal)

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