Phenotypic characterization of cryptic Diplodia species by MALDI-TOF MS and the bias of mycelium age (Articolo in rivista)

Type
Label
  • Phenotypic characterization of cryptic Diplodia species by MALDI-TOF MS and the bias of mycelium age (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1111/efp.12054 (literal)
Alternative label
  • V. Mancini, L. Dapporto, D. Baracchi, N. Luchi, S. Turillazzi and P. Capretti (2013)
    Phenotypic characterization of cryptic Diplodia species by MALDI-TOF MS and the bias of mycelium age
    in Forest pathology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • V. Mancini, L. Dapporto, D. Baracchi, N. Luchi, S. Turillazzi and P. Capretti (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1) V. Mancini, L. Dapporto, P. Capretti; 2)V. Mancini; 3) D. Baracchi, S. Turillazzi 1) Dipartimento di Biotecnologie Agrarie, Sezione di Protezione delle Piante, Universita degli Studi di Firenze, Piazzale delle Cascine, 28I 50144,Firenze, Italy; 2) Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, Sezione di Protezione delle Piante, Universita politecnica delle Marche, via Brecce Bianche, 60131 Ancona, Italy; Centro Interdipartimentale di Spettrometria di Massa dell'Universita di Firenze,Viale G. Pieraccini 6, 50139 Firenze, Italy; (literal)
Titolo
  • Phenotypic characterization of cryptic Diplodia species by MALDI-TOF MS and the bias of mycelium age (literal)
Abstract
  • Detection and identification of fungal cryptic species has been facilitated by DNA sequencing. However, the examination of some phenotypic traits is fundamental for the confirmation of genetic results. MALDI-TOF mass spectrometry has shown remarkable reliability in the recognition of species-specific phenotypic markers and has already been tested in several species of fungi. However, even though there is no direct evidence, the age of the mycelium appears to influence the composition of the metabolites detected. In this study, we demonstrate that MALDI-TOF is a reliable technique to identify suitable metabolites to distinguish three monophyletic species of Diplodia (D. pinea, D. seriata and D. scrobiculata), thus supporting recent DNA results. Nevertheless, different collections of samples over a period of 3 months after inoculation also revealed that the MALDI-TOF spectra are highly dependent on mycelial age. More importantly, the species-specific markers emerge only after 1 month of mycelial growth. The methodological and biological implications of these findings are discussed. (literal)
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