Crystallization and preliminary X-ray diffraction studies of DNA polymerase from the thermophilic archaeon Sulfolobus solfataricus (Articolo in rivista)

Type
Label
  • Crystallization and preliminary X-ray diffraction studies of DNA polymerase from the thermophilic archaeon Sulfolobus solfataricus (Articolo in rivista) (literal)
Anno
  • 1998-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1107/S0907444998002443 (literal)
Alternative label
  • Nastopoulos, V; Pisani, FM; Savino, C; Federici, L; Rossi, M; Tsernoglou, D (1998)
    Crystallization and preliminary X-ray diffraction studies of DNA polymerase from the thermophilic archaeon Sulfolobus solfataricus
    in Acta crystallographica. Section D, Biological crystallography.; MUNKSGAARD INT PUBL LTD, COPENHAGEN (Danimarca); Wiley-Blackwell, Oxford (Regno Unito)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Nastopoulos, V; Pisani, FM; Savino, C; Federici, L; Rossi, M; Tsernoglou, D (literal)
Pagina inizio
  • 1002 (literal)
Pagina fine
  • 1004 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 54 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • [ 1 ] Univ Rome La Sapienza, Dept Biochem Sci, Inst Pasteur, Fdn Cenci Bolognetti, I-00185 Rome, Italy [ 2 ] Univ Patras, Dept Chem, Patras 26500, Greece [ 3 ] CNR, Ist Biochim Prot & Enzimol, I-80125 Naples, Italy (literal)
Titolo
  • Crystallization and preliminary X-ray diffraction studies of DNA polymerase from the thermophilic archaeon Sulfolobus solfataricus (literal)
Abstract
  • The thermophilic and thermostable family B DNA polymerase from the archaeon Sulfolobus solfataricus (M-r of about 100 kDa) has been crystallized by the hanging-drop vapour-diffusion method at 294 K using ammonium sulfate as precipitant. The crystals belong to the monoclinic space group C2 with cell dimensions a = 187.4. b = 68.5, c = 125.8 Angstrom and beta = 107.8 degrees and diffract up to 2.7 Angstrom resolution on a rotating-anode X-ray source. Native data have been collected at 100 K. A heavy-atom derivative search is in progress. (literal)
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