Is NFkappa-B inhibitor alpha (IkBa) involved into oocyte-to-embryo transition? (Abstract/Poster in atti di convegno)

Type
Label
  • Is NFkappa-B inhibitor alpha (IkBa) involved into oocyte-to-embryo transition? (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Alternative label
  • Paciolla M, Boni Raffaele, Fusco F, Ursini MV, Lioi MB, Miano MG. (2009)
    Is NFkappa-B inhibitor alpha (IkBa) involved into oocyte-to-embryo transition?
    in European Society of Human Genetics 2009, Vienna, Austria, May 23-26
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Paciolla M, Boni Raffaele, Fusco F, Ursini MV, Lioi MB, Miano MG. (literal)
Note
  • Poster (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1Institute of Genetics and Biophysics \"Adriano Buzzati Traverso\" CNR, Naples, Italy; 2University of Basilicata, Potenza, Italy (literal)
Titolo
  • Is NFkappa-B inhibitor alpha (IkBa) involved into oocyte-to-embryo transition? (literal)
Abstract
  • NF-?B activity is regulated by interaction with inhibitory proteins (I?B). The canonical p65/p50 heterodimer is bound to I?B? that inhibits the NF-?B activity by masking its NLS domain and so blocking NF-?B ability to bind DNA. NF-?B-I?B? complex is continuously shuttling between nucleus and cytoplasm. Its nuclear export rate exceeds its import rate and thus the complex is generally cytoplasmic. NF-kB signalling studies during oocyte maturation have been limited. The upregulated I?B? expression has been observed in murine oocyte aging suggesting that NF-?B pathway deregulation may be related to oocyte competence. In male gamete development, NF-?B is activated in pachitene stage and in the following germinal differentiation steps triggering specialized genes expression. We analysed I?B? transcript levels in meiotic arrested bovine oocytes at different stages of maturation (diplotene of prophase I and metaphase II) and in early embryogenesis. We included three expressed oocyte genes: BMP15, GDF9, SPIN1. We examined ZAR1, specific oocyte maturation marker, whose transcript generally underwent degradation during meiosis. We observed a significant reduction of the I?B? mRNA during meiotic maturation. On the contrary, we observed that the amount of I?B? protein increased during the transition from immature to mature oocytes, while it was significantly decreased in embryos. Further analysis will be performed to establish how I?B? is regulated during developmental competence and if it is involved in human diseases such as premature aging and infertility. (literal)
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