http://www.cnr.it/ontology/cnr/individuo/prodotto/ID24211
Connected culture of murine hepatocytes and HUVEC in a multicompartmental bioreactor. (Articolo in rivista)
- Type
- Label
- Connected culture of murine hepatocytes and HUVEC in a multicompartmental bioreactor. (Articolo in rivista) (literal)
- Anno
- 2009-01-01T00:00:00+01:00 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Vozzi F; Heinrich JM; Bader A; Ahluwalia AD (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Vozzi F, Istituto di fisiologia clinica, Pisa
Heinrich JM, Laboratory of Cell Techniques and Applied Stem Cell Biology, Biocity, University of Leipzig, Leipzig, Germany
Bader A, Laboratory of Cell Techniques and Applied Stem Cell Biology, Biocity, University of Leipzig, Leipzig, Germany
Ahluwalia AD, Faculty of Engineering, Interdepartmental Research Center ''E. Piaggio,'' University of Pisa, Pisa, Italy (literal)
- Titolo
- Connected culture of murine hepatocytes and HUVEC in a multicompartmental bioreactor. (literal)
- Abstract
- A multicompartmental bioreactor was conceived and designed to mimic cross talk between cells in different
culture chambers connected only by flow, such that cell-cell interaction is mediated by soluble ligands as occurs
in the body. The system was tested with a connected culture of murine hepatocytes and human umbilical vein
endothelial cells. Metabolites such as albumin, urea, lactate and viability were monitored during the course of
the experiments and compared with monoculture conditions in the bioreactor. When the two cell types are
placed in connected culture, there is an increase in endothelial cell viability and hepatic glucose synthesis as well
as albumin and urea production, while overall lactate production in the system is downregulated. The results
show that the multicompartmental bioreactor enhances cell function, effectively combining both heterotypic
interactions with increased nutrient availability. (literal)
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- Autore CNR
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