http://www.cnr.it/ontology/cnr/individuo/prodotto/ID24205

In vitro evaluation of the PEtU-PDMS material immunocompatibility: the influence of surface topography and PDMS content (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

In vitro evaluation of the PEtU-PDMS material immunocompatibility: the influence of surface topography and PDMS content (Articolo in rivista) (literal)

Anno

2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1007/s10856-009-3823-0 (literal)

Alternative label

Spiller D, Mirtelli C, Losi P, Briganti E, Sbrana S, Counoupas C, Kull S, Tonlorenzi S, Soldani G. (2009)
In vitro evaluation of the PEtU-PDMS material immunocompatibility: the influence of surface topography and PDMS content
in Journal of materials science. Materials in medicine
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Spiller D, Mirtelli C, Losi P, Briganti E, Sbrana S, Counoupas C, Kull S, Tonlorenzi S, Soldani G. (literal)

Pagina inizio

2511 (literal)

Pagina fine

2520 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

20 (literal)

Rivista

Journal of materials science. Materials in medicine (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

10 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

12 (literal)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Laboratorio di Biomateriali - Istituto di Fisiologia Clinica del Consiglio Nazionale delle Ricerche - UOS IFC-CNR Massa Carrara Via Aurelia Sud, 54100 Massa (MS), Italy (literal)

Titolo

In vitro evaluation of the PEtU-PDMS material immunocompatibility: the influence of surface topography and PDMS content (literal)

Abstract

The aim of the present work is to evaluate the in vitro immunocompatibility of an elastomeric material with feasible applications in the cardiovascular field. In particular, since it is well known that surface chemistry and topography play a key role in the foreign body response, their influence on human monocytes was evaluated. The material, constituted by a poly(ether)urethane (PEtU) and a polydimethylsiloxane (PDMS), was synthesized to manufacture films and small-diameter vascular grafts with three different surface topographical features, smooth, rough and porous, and siloxane rates, 10, 30 and 40. Human THP-1 monocytes have been cultured for 72 h on the films and human blood has been circulating for 2 h into the grafts to assess leukocyte adhesion and cytokine releases. Materials extracts were utilized to evaluate monocyte apoptosis. Smooth films showed lower cell adhesion degrees than rough and porous ones. All the PEtU-PDMS (poly(ether)urethane-polydimethylsiloxane) films and vascular grafts induced a narrow inflammatory response, as demonstrated by slight cytokine secretion levels, in particular samples with the highest PDMS contents (30 and 40%) induced the lowest IL-1 beta secretion. Moreover, an absence of monocyte apoptosis advises that the negligible release values have not to be ascribed to material toxicity. In the end, surface topography showed to affect only monocyte adhesion while siloxane content the cytokine release. Therefore, the possibility to modify the above tested parameters during material synthesis and manufacture could allow to bound the inflammatory potency of the PEtU-PDMS devices and render them excellent candidates for cardiovascular reconstruction. (literal)

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