http://www.cnr.it/ontology/cnr/individuo/prodotto/ID24142
Study of the cross-talk between hepatocytes and endothelial cells using a novel multi-compartmental bioreactor: a comparison between connected cultures and co-cultures (Articolo in rivista)
- Type
- Label
- Study of the cross-talk between hepatocytes and endothelial cells using a novel multi-compartmental bioreactor: a comparison between connected cultures and co-cultures (Articolo in rivista) (literal)
- Anno
- 2009-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1089=ten.tea.2008.0695 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Guzzardi MA; Vozzi F; Ahluwalia AD (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Guzzardi MA, Scuola Superiore Sant'Anna, Pisa
Vozzi F, Istituto di Fisiologia Clinica, Pisa
Ahluwalia AD, Faculty of Engineering, Interdepartmental Research Center ''E.Piaggio,'' University of Pisa, Pisa (literal)
- Titolo
- Study of the cross-talk between hepatocytes and endothelial cells using a novel multi-compartmental bioreactor: a comparison between connected cultures and co-cultures (literal)
- Abstract
- The liver and other organs are connected to each other through the bloodstream. Therefore, the connection
between tissues is generally mediated by soluble molecules able to cross the endothelial wall of capillaries. We
developed a multicompartmental device, multicompartmental bioreactor (MCB), designed to mimic the connection
between different tissues in which crosstalk is mediated by soluble molecules transported through the
blood. A comparative study of the crosstalk between hepatocytes (HepG2) and endothelial cells (human umbilical
vein endothelial cells) in connected culture in the MCB and in a traditional static coculture system was
performed by analyzing glucose consumption and secretion of albumin, urea, and nitric oxide. When hepatocytes
and endothelial cells were cultured together, the production of albumin and urea increased, and the
increase was higher in the MCB than in traditional static coculture. In spite of this enhanced metabolic activity,
the crosstalk between hepatocytes and endothelial cell leads to decreased glucose consumption with respect to
hepatocytes alone, both in static and in dynamic conditions. However, the dynamic connected culture has a
higher rate of metabolite synthesis and secretion with respect to cocultures. This means a more efficient use of
energetic substrates and enhanced hepatocyte function in the MCB. (literal)
- Prodotto di
- Autore CNR
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