http://www.cnr.it/ontology/cnr/individuo/prodotto/ID24123
Sequencing and cardiac expression of Apelin in Sus Scrofa (Articolo in rivista)
- Type
- Label
- Sequencing and cardiac expression of Apelin in Sus Scrofa (Articolo in rivista) (literal)
- Anno
- 2009-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.phrs.2009.04.008 (literal)
- Alternative label
Del Ry S, Cabiati M, Raucci S, Simioniuc A, Caselli C, Prescimone T, Giannessi D (2009)
Sequencing and cardiac expression of Apelin in Sus Scrofa
in Pharmacological research (Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Del Ry S, Cabiati M, Raucci S, Simioniuc A, Caselli C, Prescimone T, Giannessi D (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- CNR Institute of Clinical Physiology, Laboratory of Cardiovascular Biochemistry, Pisa, Italy
Sector of Medicine, Scuola Superiore Sant'Anna, Pisa, Italy (literal)
- Titolo
- Sequencing and cardiac expression of Apelin in Sus Scrofa (literal)
- Abstract
- In humans, the Apelin gene is located on chromosome Xq25-26.1 and it encodes a 77-aminoacid
prepropeptide. Considerable sequence homology exists across different mammalian species. Apelin is
emerging as an important regulator of cardiovascular homeostasis but, at present, fewdata fromhumans
are available and further studies are necessary to better define its role in cardiovascular pathophysiology.
The role and function of Apelin in cardiovascular system could be reliably investigated in experimental
models devoid of confounding effects reflecting only the natural history of the disease. The pig constitutes
a model largely used in experimental pathology where it has a central role in \"in vivo\" clinical settings.
Sus Scrofa genoma is not completely sequenced and Apelin gene is still lacking. Aim of this study was to
sequence the Apelin in Sus Scrofa for future applications to molecular biology studies. Using the guanidinium
thyocyanate-phenol-chloroform method, we extracted total RNA from samples obtained from
heart of mouse and from atrium and ventricle of normal pigs. Pig Apelin mRNA was sequenced using
polymerase chain reaction primers designed from mouse consensus sequences.
A partial sequence of Sus Scrofa Apelin mRNA, 1-201 pb,was submitted to GenBank (accession number
FJ362603). The bands obtained from pig cardiac tissue shared a 99% sequence identity with Mus musculus
and 90% with Rattus norvegicus. The knowledge of Apelin sequence can be an useful starting point
for future studies devoted to better understand the possible alterations of Apelin mRNA expression in
different cardiac diseases. (literal)
- Prodotto di
- Autore CNR
Incoming links:
- Autore CNR di
- Prodotto
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi