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Expression of C-type natriuretic peptide and of its receptor NPR-B in normal and failing heart (Articolo in rivista)

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Expression of C-type natriuretic peptide and of its receptor NPR-B in normal and failing heart (Articolo in rivista) (literal)

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Del Ry S.; Cabiati M.; Lionetti V.; Emdin M.; Recchia A.; Giannessi D. (2008)
Expression of C-type natriuretic peptide and of its receptor NPR-B in normal and failing heart
in Peptides (New York, NY : 1980)
(literal)

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Del Ry S.; Cabiati M.; Lionetti V.; Emdin M.; Recchia A.; Giannessi D. (literal)

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CNR, Inst Clin Physiol, Pisa, Italy Fondazione G. Monasterio, Lab Cardiovasc Biochem, Pisa, Italy Scuola Super Sant Anna, Sector Med, Pisa, Italy New York Med Coll, Dept Physiol, Valhalla, NY, USA (literal)

Titolo

Expression of C-type natriuretic peptide and of its receptor NPR-B in normal and failing heart (literal)

Abstract

C-type natriuretic peptide (CNP) was recently found in the myocardium, but possible differences between atrium and ventricle production are so far lacking. Our aim was to evaluate, in an experimental model of pacing-induced heart failure (HF), plasma and tissue levels of CNP and mRNA expression of the peptide and of its specific receptor, NPR-B. Cardiac tissue was collected from male adult minipigs without (control, n=5) and with pacing-induced HF (n=5). Blood samples were collected at baseline and after pacing (10 min, 1, 2, 3 weeks). CNP in plasma and in cardiac extracts were determined by a radioimmunoassay, while the expression of mRNA by Real Time-PCR. Compared to control, plasma CNP was increased after 1 week of pacing stress (36.9±10.4 pg/ml vs.16.7±1.1, p=0.013, mean±sem). As to myocardial extract, at baseline CNP was found in all cardiac chambers and its content was 10-fold higher in atria than in ventricles (RA:13.7±1.9 pg/mg protein; LA:8.7±3.8; RV:1.07±0.33; LV:0.93±0.17). At 3 weeks of pacing, myocardial levels of CNP in left ventricle were higher than in controls (15.8±9.9 pg/mg protein vs.0.9±0.17, p=0.01). CNP gene expression was observed in controls and at 3 weeks of pacing. NPR-B gene expression was found in all cardiac region analyzed, and a down-regulation was observed in ventricles after HF. The co-localization of the CNP system and NPR-B suggests a possible role of CNP in HF and may prompt novel therapeutical strategies. (literal)

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