http://www.cnr.it/ontology/cnr/individuo/prodotto/ID23922
Sequencing and cardiac expression of natriuretic peptide receptor 2 (NPR-B) in Sus Scrofa (Articolo in rivista)
- Type
- Label
- Sequencing and cardiac expression of natriuretic peptide receptor 2 (NPR-B) in Sus Scrofa (Articolo in rivista) (literal)
- Anno
- 2007-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.peptides.2007.05.003 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Del Ry S.; Cabiati M.; Lionetti V.; Colotti C.; Maltinti M.; Emdin M.; Recchia F. A.; Giannessi D. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- In: Peptides, vol. 28 pp. 1390 - 1396. doi:10.1016/j.peptides.2007.05.003. Elsevier, 2007. (literal)
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- CNR Institute of Clinical Physiology, Laboratory of Cardiovascular Biochemistry, Pisa, Italy
Sector of Medicine, Scuola Superiore Sant'Anna, Pisa, Italy
Department of Physiology, New York Medical College, Valalla, NY, USA (literal)
- Titolo
- Sequencing and cardiac expression of natriuretic peptide receptor 2 (NPR-B) in Sus Scrofa (literal)
- Abstract
- The cardiovascular actions of the C-type natriuretic peptide (CNP) are mainly mediated by the interaction with natriuretic peptide receptor-B (NPR-B). The aim of this study was to identify the sequence of NPR-B in Sus Scrofa, which is not present in GenBank, to verify the expression of NPR-B in the different cardiac chambers of normal pigs and evaluate its homology with murine and human species. Using the guanidinium thyocyanate-phenolchloroform method, we extracted total RNA from samples obtained from heart of mouse and from the atrium, ventricle, and septum of normal pigs. Pig NPR-B mRNA was sequenced using polymerase chain reaction primers designed from mouse consensus sequences. Sus Scrofa natriuretic peptide receptor 2 mRNA, 1-396 bp, was submitted to GenBank (accession number DQ487044). The presence of NPR-B at mRNA level was detected in all the cardiac chambers; moreover, the bands obtained from pig cardiac tissue shared a 93% sequence homology with a region of the mouse NPR-B and a 95% sequence homology with Homo sapiens. Therefore, NPR-B sequencing provides a new tool to investigate the role of CNP under physiological and pathological conditions in the experimental and clinical setting. (literal)
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