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Devolpment of a nested PCR protocol for detection of olive-infecting viruses in crude extracts (Articolo in rivista)

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Devolpment of a nested PCR protocol for detection of olive-infecting viruses in crude extracts (Articolo in rivista) (literal)

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Pantaleo, V., Saponari, M. and Gallitelli, D. (2001)
Devolpment of a nested PCR protocol for detection of olive-infecting viruses in crude extracts
in Journal of plant pathology (Print); ETS, Pisa (Italia)
(literal)

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PV, SM: Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, CNR, Bari, Italy GD: Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi di Bari, Bari, Italy (literal)

Titolo

Devolpment of a nested PCR protocol for detection of olive-infecting viruses in crude extracts (literal)

Abstract

A reverse transcription nested polymerase chain reaction (RT-n-PCR) was developed and applied to the detection of Arabis mosaic virus (ArMV), Cherry leaf roll virus (CLRV), Olive latent virus-1 (OLV-1), Olive latent virus-2 (OLV-2), Olive latent ringspot virus (OLRSV) and Strawberry latent ringspot virus (SLRV) in naturally infected olive cuttings. The method was more sensitive and reliable than conventional RT-PCR. This increased sensitivity allowed virus detection in samples that were negative to the first round of RT-PCR amplification. Coupled with simple sample preparation from cortical scrapings of olive cuttings, this protocol should be useful in routine testing for olive viruses. (literal)

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