Granulocyte- and monocyte-platelet adhesion index in coronary and peripheral blood after extracorporeal circulation and reperfusion (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Granulocyte- and monocyte-platelet adhesion index in coronary and peripheral blood after extracorporeal circulation and reperfusion (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/cyto.b.20157 (literal)

Alternative label

• Sbrana S.; Buffa M.; Bevilacqua S.; Spiller D.; Parri M. S.; Gianetti J.; De Filippis R.; Clerico A. (2007)
  Granulocyte- and monocyte-platelet adhesion index in coronary and peripheral blood after extracorporeal circulation and reperfusion
  in Cytometry. Part B, Clinical cytometry
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Sbrana S.; Buffa M.; Bevilacqua S.; Spiller D.; Parri M. S.; Gianetti J.; De Filippis R.; Clerico A. (literal)

Pagina inizio

• 215 (literal)

Pagina fine

• 222 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 72B (literal)

Rivista

• Cytometry. Part B, Clinical cytometry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• In: Cytometry, vol. 72B (3) pp. 215 - 222. Wiley Interscience, 2007. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 72B (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Laboratory of Hematology and Flow Cytometry, CNR Institute of Clinical Physiology, Massa, Italy Cardiac Surgery Department, CNR Institute of Clinical Physiology, Massa, Italy Scuola Superiore S. Anna, Pisa, Italy (literal)

Titolo

• Granulocyte- and monocyte-platelet adhesion index in coronary and peripheral blood after extracorporeal circulation and reperfusion (literal)

Abstract

• Background: Neutrophil-granulocyte and mononuclear-cell functional changes occur during cardiopulmonary bypass and cardiovascular surgery. Leukocyte-platelet interaction, leading to generation of heterotypic co-aggregates, represents an amplification mechanism of local inflammatory response and tissue damage. Methods: Samples of 20 patients were drawn from coronary sinus before cardioplegia and after reperfusion, as well as from peripheral blood at 5 and 24 hours postoperatively. Granulocyte and monocyte expression of CD162, CD15s, CD18 and CD11b were quantified by flow cytometry at the different times. Parallel variations of leukocyte-platelet conjugates (percentages) and a derived (cell number-normalized) leukocyte-platelet adhesion index were measured using antibodies against CD45, CD14 and CD41a. The evaluation of platelet functional state was carried out using antibodies against CD62P (P-selectin) and PAC-1. Results: Monocyte and granulocyte cell number increased in coronary blood at reperfusion and in peripheral blood post-operatively. A different course characterized the changes of the leukocyte-platelet adhesion index with respect to the variations of circulating leukocyte-platelet co-aggregates . Leukocyte molecules expression showed no significant variations for CD15s on both leukocyte subsets, while a significant up-modulation for CD162 was observed on monocytes at 24 hours after extracorporeal circulation (P=0.0002), and for CD11b on granulocytes at 5 hours post-operatively (P=0.033). A loss of CD162 expression was observed at reperfusion (P=0.0038) on granulocytes, associated to a down-modulation of CD18 (P=0.0033) and CD11b (P=0.0184) in peripheral blood at 24 hours. No significant up-regulation of platelet activatory molecules was found at reperfusion, as well as post-operatively, when compared with the before-cardioplegia derived data. Conclusions: The variations of a normalized leukocyte-platelet adhesion index seem to reflect the leukocyte-platelet functional interaction more accurately than the measurements of cellular conjugates. The absence of platelet activation suggests that the leukocyte membrane modifications play a main role in controlling the formation and stability of heterotypic leukocyte-platelet co-aggregates after cardiac surgery with extracorporeal circulation. (literal)

Prodotto di

• Tassonomia clinica molecolare - Tassonomia clinica molecolare (ME.P05.003.001) (Modulo)
• Attività clinica di istituto (ME.P01.003.001) (Modulo)
• Istituto di fisiologia clinica (IFC) (Istituto)

Autore CNR

• JACOPO GIANETTI (Unità di personale interno)
• SILVERIO SBRANA (Unità di personale interno)
• ALDO CLERICO (Persona)

Insieme di parole chiave

• Keywords of "Granulocyte- and monocyte-platelet adhesion index in coronary and peripheral blood after extracorporeal circulation and reperfusion" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di fisiologia clinica (IFC) (Istituto)
• Attività clinica di istituto (ME.P01.003.001) (Modulo)
• Tassonomia clinica molecolare - Tassonomia clinica molecolare (ME.P05.003.001) (Modulo)

Autore CNR di

• JACOPO GIANETTI (Unità di personale interno)
• SILVERIO SBRANA (Unità di personale interno)
• ALDO CLERICO (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Cytometry. Part B, Clinical cytometry (Rivista)

Insieme di parole chiave di

• Keywords of "Granulocyte- and monocyte-platelet adhesion index in coronary and peripheral blood after extracorporeal circulation and reperfusion" (Insieme di parole chiave)