http://www.cnr.it/ontology/cnr/individuo/prodotto/ID23880

Glutamate-cysteine ligase polymorphism, hypertension, and male sex are associated with cardiovascular events. Biochemical and genetic characterization of Italian subpopulation (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

Glutamate-cysteine ligase polymorphism, hypertension, and male sex are associated with cardiovascular events. Biochemical and genetic characterization of Italian subpopulation (Articolo in rivista) (literal)

Anno

2007-01-01T00:00:00+01:00 (literal)

Alternative label

Campolo J.; Penco S.; Bianchi E.; Colombo L.; Parolini M.; Caruso R.; Sedda V.; Patrosso M. C.; Cighetti G.; Marocchi A.; Parodi O. (2007)
Glutamate-cysteine ligase polymorphism, hypertension, and male sex are associated with cardiovascular events. Biochemical and genetic characterization of Italian subpopulation
in The American heart journal
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Campolo J.; Penco S.; Bianchi E.; Colombo L.; Parolini M.; Caruso R.; Sedda V.; Patrosso M. C.; Cighetti G.; Marocchi A.; Parodi O. (literal)

Pagina inizio

1123 (literal)

Pagina fine

1129 (literal)

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154 (literal)

Rivista

?The ?American heart journal (Rivista)

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In: American Heart Journal, vol. 154 pp. 1123 - 1129. Elsevier, 2007. (literal)

Note

ISI Web of Science (WOS) (literal)

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CNR Clinical Physiology Institute-Milan, Cardiology Department, Niguarda Ca'Granda Hospital, Milan, Italy, Medical Genetics, Clinical Chemistry and Clinical Pathology Laboratory, Niguarda Ca' Granda Hospital, Milan, Italy, and Department of Preclinical Sciences, LITA Vialba, University of Milan, Milan, Italy. (literal)

Titolo

Glutamate-cysteine ligase polymorphism, hypertension, and male sex are associated with cardiovascular events. Biochemical and genetic characterization of Italian subpopulation (literal)

Abstract

Glutathione (GSH) is an important intravascular scavenger that protects endothelial cells from atherosclerosis. However, it is still unknown whether cardiovascular (CV) events are associated with metabolic and genetic factors, linked to GSH synthesis in an Italian subpopulation, and if a glutamate-cysteine ligase polymorphism within the catalytic subunit (GCLC) could affect blood and plasma GSH concentrations. Methods One hundred subjects, with or without CV risk factors, were enrolled to evaluate plasma and erythrocyte redox status (GSH, homocysteine, cysteine, cysteinylglycine), antioxidant vitamins (?-tocopherol and ascorbate), malondialdehyde, a lipid peroxidation product, and the presence of the GCLC-129 C/T polymorphism; an experimental hyperhomocysteinemia after methionine-induced stimulation of transsulfuration pathway was performed in 91% of enrolled subjects. Clinical, biochemical, and genetic variables were correlated with the presence of CV events (myocardial infarction, transient ischemic attacks, and stroke).Results By multiple logistic regression analysis, male sex (P = .027), hypertension (P = .001), and GCLC C/T genotype (P = .009) were the only variables associated with events. Plasma ?-tocopherol content decreased postmethionine in the T allele subjects compared with wild type (P for time × group interaction = .001). Plasma-reduced GSH level was higher in C/T than in C/C genotype subjects at both time points (P for group = .03), whereas intracellular GSH concentration did not differ between the 2 genotype groups either at baseline or postmethionine. Conclusions GCLC T allele, together with hypertension and male sex, is associated with CV events in our study population. Moreover, after stimulation of transsulfuration, intracellular GSH content is preserved in T allele subjects, probably by increases in GSH turnover and export, and consumption of ?-tocopherol. (literal)

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