A CHO mutant, UV40, that is sensitive to diverse mutagens and represents a new complementation group of mitomycin C sensitivity (Articolo in rivista)

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  • A CHO mutant, UV40, that is sensitive to diverse mutagens and represents a new complementation group of mitomycin C sensitivity (Articolo in rivista) (literal)
Anno
  • 1996-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/0921-8777(96)00014-6 (literal)
Alternative label
  • Bush DB, Zdzienicka M, Natarajan AT, Jones NJ, Overkamps WIJ, Collins A, Mitchell D, Stefanini M, Botta E, Bliss AR, Liu N, White D, van Gool AJ and Thompson LH (1996)
    A CHO mutant, UV40, that is sensitive to diverse mutagens and represents a new complementation group of mitomycin C sensitivity
    in Mutation research. DNA repair (Print); Elsevier Science Publishers, Amsterdam (Paesi Bassi)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Bush DB, Zdzienicka M, Natarajan AT, Jones NJ, Overkamps WIJ, Collins A, Mitchell D, Stefanini M, Botta E, Bliss AR, Liu N, White D, van Gool AJ and Thompson LH (literal)
Pagina inizio
  • 209 (literal)
Pagina fine
  • 221 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 363 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 3 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Stefanini, Botta: IGBE CNR Pavia Bush, Bliss, White: Armed Forces Inst Pathol, Washington, DC Zdzienicka, Natarajan, Overkamp: Leiden University NL Jones: University of Liverpool UK Mitchell:UTMD Anderson Cancer Center, Smithville,TX Collins: Rowett Res Inst, Aberdeen, Scotland Van Gool: Erasmus University, Rotterdam NL Liu, Thompson: Lawrence Livermore National Laboratory, Livermore, CA (literal)
Titolo
  • A CHO mutant, UV40, that is sensitive to diverse mutagens and represents a new complementation group of mitomycin C sensitivity (literal)
Abstract
  • A new mitomycin C (MMC)-sensitive rodent line, UV40, has been identified in the collection of ultraviolet light-(UV-) sensitive mutants of Chinese hamster ovary (CHO) cells isolated at the previous Facility for Automated Experiments in Cell Biology (FAECB). It was isolated from an UV mutant hunt using mutagenesis of AA8 cells with the DNA intercalating frameshift mutagen ICR170. It is complemented by CHO-UV-1, irs1, irs3, irs1SF, MC5, V-C8 and V-H4 with respect to its MMC sensitivity based on cell survival. Despite having approx, 4 x normal UV sensitivity and increased sensitivity to UV inhibition of DNA replication, it has near-normal incision kinetics of UV irradiated DNA, and normal (6-4) photoproducts removal. It also is not hypermutable by UV, and shows near normal levels of UV inhibition of RNA synthesis. UV40 also has approx, 11X, 10x, 5x and 2x AA8 sensitivity to MMC, ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), and X-rays, respectively. Thus, its defect apparently does not involve nucleotide excision repair but rather another process, possibly in replicating past lesions. The spontaneous chromosomal aberration frequency is elevated to 20% in UV40, and the baseline frequency of sister chromatid exchange is also similar to 4-fold increased. The phenotype of UV40 appears to differ from all other rodent mutants that have so far been described. (literal)
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