Characterization of bep1 and bep4 antigens involved in cell interaction during Paracentrotus lividus development. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Characterization of bep1 and bep4 antigens involved in cell interaction during Paracentrotus lividus development. (Articolo in rivista) (literal)

Anno

• 1992-01-01T00:00:00+01:00 (literal)

Alternative label

• Romancino DP, Ghersi G, Montana G, Bonura A, Perriera S, Di Carlo M (1992)
  Characterization of bep1 and bep4 antigens involved in cell interaction during Paracentrotus lividus development.
  in Differentiation (Lond.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Romancino DP, Ghersi G, Montana G, Bonura A, Perriera S, Di Carlo M (literal)

Pagina inizio

• 67 (literal)

Pagina fine

• 74 (literal)

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• 50 (literal)

Rivista

• Differentiation (Rivista)

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• 8 (literal)

Note

• ISI Web of Science (WOS) (literal)

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• IBIM CNR (literal)

Titolo

• Characterization of bep1 and bep4 antigens involved in cell interaction during Paracentrotus lividus development. (literal)

Abstract

• We have identified and partially characterised two antigens, extracted with 3% butanol, from Paracentrotus liuidus embryos dissociated at the blastula stage, and encoded by the cDNA clones previously described as bepl and bep4 (bep-butanol extracted proteins). The cDNA fragments containing the specific central portions of bepl and bep4 were expressed as MS2 polymerase fusion proteins in Escherichia coli. These two fusion proteins, called 1C1 (bepl) and 4A1 (bep4), were injected subcutaneously into rabbits and the corresponding polyclonal antibodies generated. Western blot analysis of proteins, extracted with 3% butanol, from sea urchin embryos at the blastula stage (b.e.p.), established that both antibodies recognize two 33 KDa proteins. Reducing and non-reducing electrophoretic conditions show that both antibodies against bepl and bep4 related proteins react also with a protein band of a molecular weight 66 KDa, indicating that these two antigens probably exist as dimers. Immunolocalization with anti 1C1 and 4A1 antibodies shows the presence of the related antigens also on the cell surface. Fab fragments of the polyclonal antibodies against 1C1 and 4A1 inhibited reaggregation of sea urchin embryonic cells, dissociated from blastula stage embryos. This prevention of reaggregation indicates that these proteins probably play a role in cell interaction during sea urchin embryonic development. (literal)

Prodotto di

• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)

Autore CNR

• ANGELA BONURA (Persona)
• GIOVANNA MONTANA (Persona)
• DANIELE ROMANCINO (Persona)
• MARTA DI CARLO (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Characterization of bep1 and bep4 antigens involved in cell interaction during Paracentrotus lividus development." (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)

Autore CNR di

• MARTA DI CARLO (Unità di personale interno)
• GIOVANNA MONTANA (Persona)
• ANGELA BONURA (Persona)
• DANIELE ROMANCINO (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Differentiation (Rivista)

Insieme di parole chiave di

• Keywords of "Characterization of bep1 and bep4 antigens involved in cell interaction during Paracentrotus lividus development." (Insieme di parole chiave)