http://www.cnr.it/ontology/cnr/individuo/prodotto/ID234436
Multidimensional analysis of poly(ethylene glycols) by size exclusion chromatography and dynamic surface tension detection (Articolo in rivista)
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- Label
- Multidimensional analysis of poly(ethylene glycols) by size exclusion chromatography and dynamic surface tension detection (Articolo in rivista) (literal)
- Anno
- 2000-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1021/ac000424b (literal)
- Alternative label
Miller, KE (Miller, KE); Bramanti, E (Bramanti, E); Prazen, BJ (Prazen, BJ); Prezhdo, M (Prezhdo, M); Skogerboe, KJ (Skogerboe, KJ); Synovec, RE (Synovec, RE) (2000)
Multidimensional analysis of poly(ethylene glycols) by size exclusion chromatography and dynamic surface tension detection
in Analytical chemistry (Online); ACS, American chemical society, Washington, DC (Stati Uniti d'America)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Miller, KE (Miller, KE); Bramanti, E (Bramanti, E); Prazen, BJ (Prazen, BJ); Prezhdo, M (Prezhdo, M); Skogerboe, KJ (Skogerboe, KJ); Synovec, RE (Synovec, RE) (literal)
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Univ Washington, Dept Chem, Ctr Process Analyt Chem, Seattle, WA 98195 USA
CNR, Natl Res Council, Inst Instrumental Anal Chem, I-56010 Pisa, Italy
Seattle Univ, Dept Chem, Seattle, WA 98122 USA (literal)
- Titolo
- Multidimensional analysis of poly(ethylene glycols) by size exclusion chromatography and dynamic surface tension detection (literal)
- Abstract
- Substantial improvements in a multidimensional dynamic surface tension detector (DSTD) are presented. Rapid, online calibration and measurement of the dynamic surface tension for high-performance liquid chromatography separations is achieved. Dynamic surface tension is determined by measuring the differential. pressure across the liquid-air interface of repeatedly growing and detaching drops. Continuous surface tension measurement throughout the entire drop growth (50 ms to 2 s) is achieved, for each eluting drop, providing insight into the kinetic behavior of molecular orientation processes at the liquid-air interface. Three-dimensional data are obtained, with surface tension first concerted to surface pressure, which is plotted as a function of elution time axis versus drop time axis. Two key innovations will be reported. First, a novel calibration procedure is described and implemented. Differential pressure signals from three drops (mobile phase, standard in mobile phase, and analyte in mobile phase) are utilized to make the dynamic surface tension measurement, thereby eliminating the need for optical imaging, and viscosity and hydrostatic pressure corrections, as required by other methods. Only pressure signals from one mobile-phase drop and one standard drop pressure signal are required, while the analyte drop pressure signal is measured along the chromatographic time axis. Second, corrections for drop elongation are not required, because the drops are precisely detached bq an air burst actuation method in a regime were the surface tension forces significantly dominate gravitational forces. Drops that would fall with a volume of similar to 10 mu L due to gravity are precisely and repeatedly detached earlier at a volume of 2 mu L. The sensitivity and unique selectivity of the DSTD opens up new possibilities in the analysis of small molecular weight polymers of varying degrees of surface activity, as illustrated for the size-exclusion chromatography analyses of complex poly(ethylene glycol) (PEG) samples. Using partial least squares for data analysis, polydispersity of complex PEG samples is determined at a relative precision of similar to 1%. (literal)
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