EGFR mutation detection by microfluidic technology: a validation study. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• EGFR mutation detection by microfluidic technology: a validation study. (Articolo in rivista) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Alternative label

• Malapelle U, Russo S, Pepe F, Sgariglia R, De Luca C, Bellevicine C, Pallante P, Troncone G. (2013)
  EGFR mutation detection by microfluidic technology: a validation study.
  in Journal of Clinical Pathology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Malapelle U, Russo S, Pepe F, Sgariglia R, De Luca C, Bellevicine C, Pallante P, Troncone G. (literal)

Rivista

• Journal of Clinical Pathology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Dipartimento di Sanità Pubblica, Università di Napoli Federico II, Napoli, Italia. (literal)

Titolo

• EGFR mutation detection by microfluidic technology: a validation study. (literal)

Abstract

• Advanced non-small cell lung cancer samples are tested for epidermal growth factor receptor (EGFR) gene mutations. Their detection by direct sequencing is time-consuming. Conversely, the length analysis of fluorescently labelled PCR products is easier. To avoid labelled primers and the automated capillary electrophoresis apparatus, we validated a fast and sensitive chip-based microfluidic technology. The limit of detection of fragment length assay on microfluidic device was 5%, more sensitive than direct sequencing (12.5%). The novel methodology showed high accuracy in the analysis of samples whose mutational status was known. The accuracy in quantifying mutated alleles (mA) was evaluated by PCR products subcloning; the mA% provided by direct sequencing of subcloned PCR products showed a close correlation with the mA% provided by the microfluidic technology for both exon 19 (R2=0.9) and 21 (R2=0.9). Microfluidic-based on-chip electrophoresis makes EGFR testing more rapid, sensitive and cost-effective. (literal)

Prodotto di

• Institute Experimental Endocrinology and Oncology \"Gaetano Salvatore\" (IEOS) (Istituto)
• Ruolo delle proteine cromatiniche nella tumorigenesi (SV.P15.001.002) (Modulo)

Autore CNR

• PIERLORENZO PALLANTE (Persona)

Incoming links:

Prodotto

• Institute Experimental Endocrinology and Oncology \"Gaetano Salvatore\" (IEOS) (Istituto)
• Ruolo delle proteine cromatiniche nella tumorigenesi (SV.P15.001.002) (Modulo)

Autore CNR di

• PIERLORENZO PALLANTE (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of Clinical Pathology (Rivista)