Leveraging on nanomechanical sensors to single out active small ligands for beta(2)-microglobulin (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Leveraging on nanomechanical sensors to single out active small ligands for beta(2)-microglobulin (Articolo in rivista) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.snb.2012.09.032 (literal)

Alternative label

• Oliviero, Giulio; Chiari, Marcella; De Lorenzi, Ersilia; Colombo, Raffaella; Cretich, Marina; Damin, Francesco; Federici, Stefania; Depero, Laura E.; Bergese, Paolo (2013)
  Leveraging on nanomechanical sensors to single out active small ligands for beta(2)-microglobulin
  in Sensors and actuators. B, Chemical (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Oliviero, Giulio; Chiari, Marcella; De Lorenzi, Ersilia; Colombo, Raffaella; Cretich, Marina; Damin, Francesco; Federici, Stefania; Depero, Laura E.; Bergese, Paolo (literal)

Pagina inizio

• 1026 (literal)

Pagina fine

• 1031 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 176 (literal)

Rivista

• Sensors and actuators. B, Chemical (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 6 (literal)

Note

• ISI Web of Science (WoS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• University of Brescia; University of Brescia; Consiglio Nazionale delle Ricerche (CNR); University of Pavia (literal)

Titolo

• Leveraging on nanomechanical sensors to single out active small ligands for beta(2)-microglobulin (literal)

Abstract

• A nanomechanical biosensor based on microcantilevers was implemented to test low molecular weight (small) compounds for their ability to stabilize beta(2)-microglobulin (beta(2)-m) in its native conformation. beta(2)-m was immobilized on the top face of silicon microcantilevers and it was demonstrated that pH induced unfolding of the immobilized beta(2)-m drives a specific microcantilever bending. This beta(2)-m microcantilever assay was then implemented to probe the effect of a pilot set small ligands on beta(2)-m conformational stability. Among the tested ligands, congo red was the only one able to protect beta(2)-m from unfolding, that is known to be the primary trigger of its self-polymerization into fibrils and in turn of the onset of amyloidosis. These findings disclose the high potentiality of nanomechanical sensors in the field of protein conformation related diseases, as they bring the unique advantage of directly screening compounds for their specific pharmacological activity rather than for generic binding preferences, effectively shortcutting the identification of the active ones. (C) 2012 Elsevier B. V. All rights reserved. (literal)

Prodotto di

• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)
• Sintesi di materiali funzionali e sviluppo di sistemi analitici miniaturizzati (PM.P06.003.001) (Modulo)

Autore CNR

• MARCELLA CHIARI (Unità di personale interno)
• FRANCESCO DAMIN (Persona)
• MARINA CRETICH (Persona)

Insieme di parole chiave

• Parole chiave di "Leveraging on nanomechanical sensors to single out active small ligands for beta(2)-microglobulin" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• MARINA CRETICH (Persona)
• MARCELLA CHIARI (Unità di personale interno)
• FRANCESCO DAMIN (Persona)

Prodotto

• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)
• Sintesi di materiali funzionali e sviluppo di sistemi analitici miniaturizzati (PM.P06.003.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Sensors and actuators. B, Chemical (Rivista)

Insieme di parole chiave di

• Parole chiave di "Leveraging on nanomechanical sensors to single out active small ligands for beta(2)-microglobulin" (Insieme di parole chiave)