http://www.cnr.it/ontology/cnr/individuo/prodotto/ID228539
Assessment of genetic variation in a working collection of Vigna vexillata (L.) A. Rich. by isozyme and RAPD analyses (Articolo in rivista)
- Type
- Label
- Assessment of genetic variation in a working collection of Vigna vexillata (L.) A. Rich. by isozyme and RAPD analyses (Articolo in rivista) (literal)
- Anno
- 1998-01-01T00:00:00+01:00 (literal)
- Alternative label
Spinosa A., Pignone D., Sonnante G. (1998)
Assessment of genetic variation in a working collection of Vigna vexillata (L.) A. Rich. by isozyme and RAPD analyses
in Genetic resources and crop evolution (Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Spinosa A., Pignone D., Sonnante G. (literal)
- Pagina inizio
- Pagina fine
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- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Scopu (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Titolo
- Assessment of genetic variation in a working collection of Vigna vexillata (L.) A. Rich. by isozyme and RAPD analyses (literal)
- Abstract
- Genetic variation based on isozyme and RAPD analyses was investigated in 47 and 34 accessions respectively
of Vigna vexillata from different geographical origins and belonging to three botanical varieties. A total of 9
enzyme systems were studied, accounting for 14 putative loci, 8 of which were polymorphic. The analysis of
genetic diversity revealed a low level of within accession variation (HS=0.013), while between accession diversity
(DST ) was 0.120. Coefficient of gene differentiation (GST ) was 0.905, indicating that most variation was among
accessions. Nei's genetic distances were calculated on the basis of allelic frequencies and a UPGMA dendrogram
was constructed.
Twenty arbitrary 10mer
oligonucleotideswere used in RAPD analysis. Amplification profiles disclosed a higher
level of polymorphism than isozymes.Based on amplification patterns, the similarity index of Jaccardwas calculated
and a dendrogram constructed on the basis of the similarity matrix. The final clustering based on RAPD data was
similar to the one obtained using isozyme allelic frequencies.
The classification in botanical varieties did not reflect the allelic constitution of the different samples. On the
other hand, referring to geographical origin, most accessions from Africa and from Latin America were distributed
respectively in two distinct clusters in the dendrogram. This grouping might also reflect the differences observed in
the germination behaviour of V. vexillata from the two continents. (literal)
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