Comparison of molecular cytogenetic and genetic analyses in accessions of the two biotypes of Vicia benghalensis L. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Comparison of molecular cytogenetic and genetic analyses in accessions of the two biotypes of Vicia benghalensis L. (Articolo in rivista) (literal)

Anno

• 1997-01-01T00:00:00+01:00 (literal)

Alternative label

• Galasso I., Sonnante G., Tota D.G., Pignone D. (1997)
  Comparison of molecular cytogenetic and genetic analyses in accessions of the two biotypes of Vicia benghalensis L.
  in Annals of botany (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Galasso I., Sonnante G., Tota D.G., Pignone D. (literal)

Pagina inizio

• 311 (literal)

Pagina fine

• 317 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 79 (literal)

Rivista

• Annals of botany (Rivista)

Note

• ISI Web of Science (WOS) (literal)
• Scopus (literal)
• Google Scholar (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR-IBBA CNR-IGV (literal)

Titolo

• Comparison of molecular cytogenetic and genetic analyses in accessions of the two biotypes of Vicia benghalensis L. (literal)

Abstract

• InVicia benghalensis two different biotypes, named A and B, had been observed which differed for several cytological, genetical and biochemical characters. In the present study 27 accessions of this species belonging to the two biotypes were investigated using different molecular analyses, supported by an attempt to produce hybrids between selected accessions. RAPD fingerprinting of the 27 accessions demonstrated that accessions belonging to biotype A show a high degree of genetic similarity, while the opposite is true for the accessions of biotype B. The total genomic DNA from one reference accession was used as a probe to Southern blots of the DNA extracted from all the accessions in use. This analysis demonstrated that cross hybridization among the DNA of the two biotypes occurs only to a limited extent. The chromosomal localization of 18S-5.8S-25S and 5S rRNA gene clusters was determined byin situ hybridization. The results indicate that the two biotypes differ in the position of one of the 5S rRNA gene clusters. This indicates a major chromosomal rearrangement. Cell synchronization experiments on two reference accessions suggested that the two biotypes might differ in the duration of the single phases of the cell cycle. Finally, in crossing experiments three pods were obtained which developed for only a few days and proved to bear no viable seeds. These results confirm the existence of two divergent gene-pools and demonstrate the reproductive barrier between the two biotypes, thus suggesting the possibility of a specific ranking. (literal)

Prodotto di

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)

Autore CNR

• GABRIELLA SONNANTE (Persona)
• DOMENICO PIGNONE (Unità di personale interno)
• INCORONATA GALASSO (Persona)

Insieme di parole chiave

• Parole chiave di "Comparison of molecular cytogenetic and genetic analyses in accessions of the two biotypes of Vicia benghalensis L." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)

Autore CNR di

• INCORONATA GALASSO (Persona)
• GABRIELLA SONNANTE (Persona)
• DOMENICO PIGNONE (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Annals of botany (Rivista)

Insieme di parole chiave di

• Parole chiave di "Comparison of molecular cytogenetic and genetic analyses in accessions of the two biotypes of Vicia benghalensis L." (Insieme di parole chiave)