Protein Crystal Growth in the Advance Protein Crystallisation Facility on the LMS Mission: a Comparison of Sulfolobus solfataricus Alchool Dehydrogenase Crystals Grown on the Ground and in Microgravity (Articolo in rivista)

Type
Label
  • Protein Crystal Growth in the Advance Protein Crystallisation Facility on the LMS Mission: a Comparison of Sulfolobus solfataricus Alchool Dehydrogenase Crystals Grown on the Ground and in Microgravity (Articolo in rivista) (literal)
Anno
  • 1998-01-01T00:00:00+01:00 (literal)
Alternative label
  • Esposito, L., Sica, F., Sorrentino, G., Berisio, R., Carotenuto, L., Giordano, A., Raia, C., Rossi, M., Lamzin, V.S., Wilson, K.S., Zagari, A. (1998)
    Protein Crystal Growth in the Advance Protein Crystallisation Facility on the LMS Mission: a Comparison of Sulfolobus solfataricus Alchool Dehydrogenase Crystals Grown on the Ground and in Microgravity
    in Acta crystallographica. Section D, Biological crystallography.
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Esposito, L., Sica, F., Sorrentino, G., Berisio, R., Carotenuto, L., Giordano, A., Raia, C., Rossi, M., Lamzin, V.S., Wilson, K.S., Zagari, A. (literal)
Pagina inizio
  • 386 (literal)
Pagina fine
  • 390 (literal)
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  • 54 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Centro di Studio di Biocristallografia, CNR, Via Mezzocannone 4, 80134 Naples, Italy, Dipartimento di Chimica, Università di Napoli , Via Mezzocannone 4, 80134 Naples, Italy Microgravity Advanced Research & Support Center (MARS), Via Comunale Tavernola, 80144 Naples, Italy Istituto di Biochimica delle Proteine ed Enzimologia, CNR, Via Marconi 10 Naples, Italy European Molecular Biology Laboratory (EMBL) c/o DESY, Notkestrasse 85, D-22603 Hamburg (literal)
Titolo
  • Protein Crystal Growth in the Advance Protein Crystallisation Facility on the LMS Mission: a Comparison of Sulfolobus solfataricus Alchool Dehydrogenase Crystals Grown on the Ground and in Microgravity (literal)
Abstract
  • Crystals of alcohol dehydrogenase from Sulfolobus solfataricus were grown in the Advanced Protein Crystallization Facility during the Life and Microgravity Sciences Spacelab mission on the US Space Shuttle. Large diffracting crystals were obtained by dialysis, whereas only poor-quality crystals were obtained by vapour diffusion. The quality of both the microgravity and ground-based crystals was analysed by X-ray diffraction. There was some improvement in terms of size and diffraction resolution limit for the microgravity crystals. However, thc twinning observed in the Earthgrown crystals was also present for those grown in microgravity. (literal)
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