http://www.cnr.it/ontology/cnr/individuo/prodotto/ID226426
A 54 kDa protein specifically associates the 3'UTR of three maternal mRNAs to the cytoskeleton of the animal of the P. lividus egg (Articolo in rivista)
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- A 54 kDa protein specifically associates the 3'UTR of three maternal mRNAs to the cytoskeleton of the animal of the P. lividus egg (Articolo in rivista) (literal)
- Anno
- 1997-01-01T00:00:00+01:00 (literal)
- Alternative label
Montana G., Bonura A, Sbisà E. Romancino DP Di Carlo M. (1997)
A 54 kDa protein specifically associates the 3'UTR of three maternal mRNAs to the cytoskeleton of the animal of the P. lividus egg
in The FEBS journal (Print); Blackwell, Oxford (Regno Unito)
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- Montana G., Bonura A, Sbisà E. Romancino DP Di Carlo M. (literal)
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- Titolo
- A 54 kDa protein specifically associates the 3'UTR of three maternal mRNAs to the cytoskeleton of the animal of the P. lividus egg (literal)
- Abstract
- Bep mRNAs, i.e., maternal messengers coding for cell surface proteins, are localized in the animal
part of Paracertrotus lividus egg and embryos. Here we have examined the involvement of the cytoskeleton
in asymmetric distribution of bep3 mRNA. Moreover, in order to understand whether and how cisand
trans-acting factors are necessary for bep3 mRNA localization, we have looked for in vitro-specific
interactions between egg proteins and bep3 mRNA. By northwestern assay we have identified a 54-kDa
protein that binds to the 3'UTR of bep3 mRNA. This 54-kDa protein also permits association of 3'UTR
of bep3 with cytoskeleton elements, indicating its involvement in the localization process. Binding of 54-
kDa protein to 3'UTR of bepl and bep4 has also been demonstrated, suggesting that a binding motif is
shared with these other two mRNAs of the same gene family. Northwestern analyses carried out utilizing
proteins extracted from different developmental stages indicate that the 54-kDa protein is the only protein
able to bind to the 3'UTR of bep3. (literal)
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