A spectroscopic study of the wavelength-dependent photoisomerizations of bilirubins bound to human serum albumin (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• A spectroscopic study of the wavelength-dependent photoisomerizations of bilirubins bound to human serum albumin (Articolo in rivista) (literal)

Anno

• 2005-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1088/1464-4258/7/12/006 (literal)

Alternative label

• Marina Mazzoni; Giovanni Agati; Riccardo Pratesi; Maurizio Persico (2005)
  A spectroscopic study of the wavelength-dependent photoisomerizations of bilirubins bound to human serum albumin
  in Journal of optics. A, Pure and applied optics (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Marina Mazzoni; Giovanni Agati; Riccardo Pratesi; Maurizio Persico (literal)

Pagina inizio

• 742 (literal)

Pagina fine

• 747 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://dx.doi.org/ (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 7 (literal)

Rivista

• Journal of optics. A, Pure and applied optics (Rivista)

Note

• Google Scholar (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Fisica Applicata 'Nello Carrara' del Consiglio Nazionale delle Ricerche, Via Madonna del Piano, I-50019 Sesto Fiorentino, Italy. Dipartimento di Fisica, Università di Firenze, via G Sansone 1, I-50019 Sesto Fiorentino, Italy. Dipartimento di Chimica e Chimica Industriale, Università di Pisa, via Risorgimento 35, I-56126 Pisa, Italy. (literal)

Titolo

• A spectroscopic study of the wavelength-dependent photoisomerizations of bilirubins bound to human serum albumin (literal)

Abstract

• The wavelength-dependent photoisomerizations of the asymmetric bilirubin BR-IX? and of the symmetric bilirubin-III? (BR-III) and mesobilirubin-XIII? (MBR-XIII) bound to human serum albumin (HSA) in aqueous solution were analysed with the help of an exciton coupling model. The modelling was based on the absorption and circular dichroism (CD) spectra (bisignate Cotton effect). Time-dependent density functional theory (TD-DFT) of the free BR-IX molecule suggested the presence of two main bands of exciton coupling character in the blue region of the spectrum, and other weaker bands of charge transfer character at longer wavelengths. These peculiarities were taken into account to fit the photoisomerization quantum yields in the blue-green region as functions of the wavelength, obtaining the bandshape of the exciton coupling bands from the experimental CD spectra. The other excitons were extracted from the decomposition of the band resulting from the difference between the absorption spectrum and the sum (normalized-to-absorption) of the two CD excitons. We expressed photoisomerization quantum yields in terms of the sum of the contributions to photon absorption deriving from all the exciton states normalized to total absorption. For all the reversible photoprocesses of bilirubins and for the irreversible one of BR-IX? in HSA (i.e. lumirubin formation), we give reliable mean values of the individual state excitation probabilities and photoisomerization efficiencies in the pigment-protein complex. (literal)

Prodotto di

• Sviluppo di strumentazione per lo spazio e l'ambiente (MD.P03.018.001) (Modulo)
• Institute of applied physics \"Nello Carrara\" (IFAC) (Istituto)

Autore CNR

• MARINA MAZZONI (Unità di personale interno)
• GIOVANNI AGATI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "A spectroscopic study of the wavelength-dependent photoisomerizations of bilirubins bound to human serum albumin" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• GIOVANNI AGATI (Unità di personale interno)
• MARINA MAZZONI (Unità di personale interno)

Prodotto

• Institute of applied physics \"Nello Carrara\" (IFAC) (Istituto)
• Sviluppo di strumentazione per lo spazio e l'ambiente (MD.P03.018.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of optics. A, Pure and applied optics (Rivista)

Insieme di parole chiave di

• Keywords of "A spectroscopic study of the wavelength-dependent photoisomerizations of bilirubins bound to human serum albumin" (Insieme di parole chiave)